Gene description for RNF14
Gene name ring finger protein 14
Gene symbol RNF14
Other names/aliases ARA54
HFB30
HRIHFB2038
TRIAD2
Species Homo sapiens
 Database cross references - RNF14
ExoCarta ExoCarta_9604
Vesiclepedia VP_9604
Entrez Gene 9604
HGNC 10058
MIM 605675
UniProt Q9UBS8  
 RNF14 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RNF14
Molecular Function
    transcription coactivator activity GO:0003713 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin-like protein transferase activity GO:0019787 IDA
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    nuclear androgen receptor binding GO:0050681 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    signal transduction GO:0007165 TAS
    protein ubiquitination GO:0016567 IEP
    androgen receptor signaling pathway GO:0030521 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    regulation of androgen receptor signaling pathway GO:0060765 IDA
    regulation of canonical Wnt signaling pathway GO:0060828 IDA
    rescue of stalled ribosome GO:0072344 IDA
    protein K6-linked ubiquitination GO:0085020 IDA
    protein-RNA covalent cross-linking repair GO:0160127 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified RNF14 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNF14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pcdh1  
Affinity Capture-MS Mus musculus
2 UBE2W  
Two-hybrid Homo sapiens
3 VDR  
Two-hybrid Homo sapiens
4 TNFAIP3 7128
Two-hybrid Homo sapiens
5 AKT1 207
Affinity Capture-MS Homo sapiens
6 ECT2 1894
Affinity Capture-MS Homo sapiens
7 UBE2V1 7335
Two-hybrid Homo sapiens
8 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNF14 9604
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
10 UBE2E3 10477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 CTNNB1 1499
Affinity Capture-Western Homo sapiens
12 HNF1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 DACH1  
Two-hybrid Homo sapiens
15 UBE2E1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 HNRNPA1 3178
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 PNPLA7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 TAGLN 6876
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 PHF7  
Two-hybrid Homo sapiens
20 UBE2D2 7322
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
21 TCF4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 UBE2D4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 UBE2E2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
24 UBE2L3 7332
Reconstituted Complex Homo sapiens
25 OSBPL2 9885
Affinity Capture-MS Homo sapiens
26 TCF3  
Affinity Capture-Western Homo sapiens
27 FAM46A  
Two-hybrid Homo sapiens
28 AR 367
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
29 LEF1  
Affinity Capture-Western Homo sapiens
30 DET1  
Affinity Capture-Western Homo sapiens
31 POLRMT 5442
Affinity Capture-MS Homo sapiens
32 UBE2U  
Two-hybrid Homo sapiens
33 UBE2D3 7323
Two-hybrid Homo sapiens
34 UBE2D1 7321
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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