Gene description for SPRED1
Gene name sprouty-related, EVH1 domain containing 1
Gene symbol SPRED1
Other names/aliases NFLS
PPP1R147
hSpred1
spred-1
Species Homo sapiens
 Database cross references - SPRED1
ExoCarta ExoCarta_161742
Vesiclepedia VP_161742
Entrez Gene 161742
HGNC 20249
MIM 609291
UniProt Q7Z699  
 SPRED1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for SPRED1
Molecular Function
    phosphatase binding GO:0019902 IDA
    protein binding GO:0005515 IPI
    stem cell factor receptor binding GO:0005173 ISS
    protein serine/threonine kinase inhibitor activity GO:0030291 ISS
    protein kinase binding GO:0019901 IPI
Biological Process
    positive regulation of DNA damage response, signal transduction by p53 class mediator GO:0043517 ISS
    inactivation of MAPK activity GO:0000188 ISS
    negative regulation of phosphatase activity GO:0010923 IDA
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IMP
    regulation of protein deacetylation GO:0090311 ISS
    multicellular organismal development GO:0007275 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    caveola GO:0005901 IEA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified SPRED1 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for SPRED1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPRED1 is involved
No pathways found





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