Gene description for CARS2
Gene name cysteinyl-tRNA synthetase 2, mitochondrial (putative)
Gene symbol CARS2
Other names/aliases cysRS
Species Homo sapiens
 Database cross references - CARS2
ExoCarta ExoCarta_79587
Vesiclepedia VP_79587
Entrez Gene 79587
HGNC 25695
MIM 612800
UniProt Q9HA77  
 CARS2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CARS2
Molecular Function
    cysteine-tRNA ligase activity GO:0004817 IBA
    cysteine-tRNA ligase activity GO:0004817 IMP
    ATP binding GO:0005524 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    cysteinyl-tRNA aminoacylation GO:0006423 IBA
    cysteinyl-tRNA aminoacylation GO:0006423 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified CARS2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FABP3 2170
Co-fractionation Homo sapiens
2 MTERF3  
Proximity Label-MS Homo sapiens
3 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 SLIRP 81892
Proximity Label-MS Homo sapiens
5 BPHL 670
Co-fractionation Homo sapiens
6 HSD17B4 3295
Co-fractionation Homo sapiens
7 PMPCB 9512
Proximity Label-MS Homo sapiens
8 TACO1  
Proximity Label-MS Homo sapiens
9 FASTKD2  
Proximity Label-MS Homo sapiens
10 TSFM 10102
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
11 AUH 549
Proximity Label-MS Homo sapiens
12 RGCC 28984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MRRF  
Proximity Label-MS Homo sapiens
14 TBRG4 9238
Proximity Label-MS Homo sapiens
15 MRPS12  
Proximity Label-MS Homo sapiens
16 MTIF2 4528
Proximity Label-MS Homo sapiens
17 TRMT61B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MDH2 4191
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
19 CLPP 8192
Proximity Label-MS Homo sapiens
20 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 MTIF3  
Proximity Label-MS Homo sapiens
22 LONP1 9361
Proximity Label-MS Homo sapiens
23 RPA2 6118
Proximity Label-MS Homo sapiens
24 WBP5  
Affinity Capture-MS Homo sapiens
25 ANXA1 301
Co-fractionation Homo sapiens
26 MRPL18 29074
Affinity Capture-MS Homo sapiens
27 MTG2  
Proximity Label-MS Homo sapiens
28 C6orf203  
Proximity Label-MS Homo sapiens
29 PTEN 5728
Synthetic Growth Defect Homo sapiens
30 FASTKD5  
Proximity Label-MS Homo sapiens
31 EGFR 1956
Affinity Capture-MS Homo sapiens
32 PPIA 5478
Co-fractionation Homo sapiens
33 ICT1 3396
Proximity Label-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 DHX30 22907
Proximity Label-MS Homo sapiens
36 CFL1 1072
Co-fractionation Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 C21orf33  
Proximity Label-MS Homo sapiens
39 MTRF1  
Proximity Label-MS Homo sapiens
40 BLM 641
Synthetic Growth Defect Homo sapiens
41 SSBP1 6742
Proximity Label-MS Homo sapiens
42 MRPS26 64949
Proximity Label-MS Homo sapiens
43 C17orf80 55028
Proximity Label-MS Homo sapiens
44 TUFM 7284
Proximity Label-MS Homo sapiens
45 LRPPRC 10128
Proximity Label-MS Homo sapiens
46 COCH 1690
Affinity Capture-MS Homo sapiens
47 C12orf65  
Proximity Label-MS Homo sapiens
48 RPA3 6119
Proximity Label-MS Homo sapiens
49 HSCB 150274
Proximity Label-MS Homo sapiens
50 C1QBP 708
Proximity Label-MS Homo sapiens
51 GFM1 85476
Proximity Label-MS Homo sapiens
52 AARS2  
Proximity Label-MS Homo sapiens
53 TFAM 7019
Proximity Label-MS Homo sapiens
54 TIMM8A 1678
Co-fractionation Homo sapiens
55 PMPCA 23203
Proximity Label-MS Homo sapiens
56 COX8A  
Proximity Label-MS Homo sapiens
57 PTRHD1 391356
Co-fractionation Homo sapiens
58 CCDC109B 55013
Proximity Label-MS Homo sapiens
59 RPUSD3  
Proximity Label-MS Homo sapiens
60 GRSF1 2926
Proximity Label-MS Homo sapiens
61 ACAD9 28976
Proximity Label-MS Homo sapiens
62 PDHA1 5160
Proximity Label-MS Homo sapiens
63 C8orf82  
Proximity Label-MS Homo sapiens
64 SLC25A12 8604
Proximity Label-MS Homo sapiens
65 TRUB2  
Proximity Label-MS Homo sapiens
66 METTL17  
Proximity Label-MS Homo sapiens
67 MRPL11 65003
Proximity Label-MS Homo sapiens
68 MTRF1L  
Proximity Label-MS Homo sapiens
69 CAPN1 823
Affinity Capture-MS Homo sapiens
70 METTL15  
Proximity Label-MS Homo sapiens
71 LYPD2  
Affinity Capture-MS Homo sapiens
72 TMEM70  
Proximity Label-MS Homo sapiens
73 BIRC6 57448
Affinity Capture-MS Homo sapiens
74 MCUR1 63933
Proximity Label-MS Homo sapiens
75 DDX28  
Proximity Label-MS Homo sapiens
76 EXD2  
Proximity Label-MS Homo sapiens
77 OGT 8473
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CARS2 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Mitochondrial tRNA aminoacylation IEA Reactome
Translation IEA Reactome
tRNA Aminoacylation IEA Reactome





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