Gene description for TIMM8A
Gene name translocase of inner mitochondrial membrane 8 homolog A (yeast)
Gene symbol TIMM8A
Other names/aliases DDP
DDP1
DFN1
MTS
TIM8
Species Homo sapiens
 Database cross references - TIMM8A
ExoCarta ExoCarta_1678
Vesiclepedia VP_1678
Entrez Gene 1678
HGNC 11817
MIM 300356
UniProt O60220  
 TIMM8A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TIMM8A
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    nervous system development GO:0007399 TAS
    protein insertion into mitochondrial inner membrane GO:0045039 IMP
    protein insertion into mitochondrial inner membrane GO:0045039 NAS
    protein insertion into mitochondrial inner membrane GO:0045039 TAS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial intermembrane space GO:0005758 NAS
    mitochondrial intermembrane space protein transporter complex GO:0042719 IPI
 Experiment description of studies that identified TIMM8A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TIMM8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAVER2  
Affinity Capture-MS Homo sapiens
2 ZNF282  
Affinity Capture-MS Homo sapiens
3 C6orf15  
Affinity Capture-MS Homo sapiens
4 C1QTNF2  
Two-hybrid Homo sapiens
5 PHB2 11331
Proximity Label-MS Homo sapiens
6 GFER  
Affinity Capture-MS Homo sapiens
7 PDYN  
Affinity Capture-MS Homo sapiens
8 SLC25A12 8604
Proximity Label-MS Homo sapiens
9 ERCC6L 54821
Affinity Capture-MS Homo sapiens
10 AMBRA1  
Affinity Capture-MS Homo sapiens
11 SOX6  
Affinity Capture-MS Homo sapiens
12 CDA 978
Co-fractionation Homo sapiens
13 APOO 79135
Proximity Label-MS Homo sapiens
14 STAM2 10254
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 UBE2L6 9246
Affinity Capture-Western Homo sapiens
16 ANKRD9  
Affinity Capture-MS Homo sapiens
17 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
18 TIMM10  
Co-fractionation Homo sapiens
19 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
20 PPIA 5478
Co-fractionation Homo sapiens
21 XRCC6BP1  
Affinity Capture-MS Homo sapiens
22 OCIAD1 54940
Affinity Capture-MS Homo sapiens
23 LYZL6  
Affinity Capture-MS Homo sapiens
24 MICU2 221154
Affinity Capture-MS Homo sapiens
25 CARS2 79587
Co-fractionation Homo sapiens
26 DIABLO 56616
Proximity Label-MS Homo sapiens
27 KRT15 3866
Two-hybrid Homo sapiens
28 USP30  
Affinity Capture-MS Homo sapiens
29 ARNT 405
Affinity Capture-MS Homo sapiens
30 TIMM9  
Co-fractionation Homo sapiens
31 ARID3A  
Two-hybrid Homo sapiens
32 PRKCDBP 112464
Two-hybrid Homo sapiens
33 JOSD1  
Affinity Capture-MS Homo sapiens
34 TIMMDC1  
Affinity Capture-MS Homo sapiens
35 PPA2 27068
Co-fractionation Homo sapiens
36 FOXQ1  
Affinity Capture-MS Homo sapiens
37 XRCC3  
Affinity Capture-MS Homo sapiens
38 OIP5  
Two-hybrid Homo sapiens
39 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PAQR4  
Affinity Capture-MS Homo sapiens
41 ATP8  
Affinity Capture-MS Homo sapiens
42 TACC3  
Affinity Capture-MS Homo sapiens
43 TIMM13 26517
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
44 CDT1  
Affinity Capture-MS Homo sapiens
45 FAM210B  
Affinity Capture-MS Homo sapiens
46 C22orf39  
Affinity Capture-MS Homo sapiens
47 STAM 8027
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
48 COX4I1 1327
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TIMM8A is involved
PathwayEvidenceSource
Mitochondrial protein import IEA Reactome
Protein localization IEA Reactome





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