Gene description for PNPLA8
Gene name patatin-like phospholipase domain containing 8
Gene symbol PNPLA8
Other names/aliases IPLA2(GAMMA)
IPLA2-2
IPLA2G
MMLA
iPLA2gamma
Species Homo sapiens
 Database cross references - PNPLA8
ExoCarta ExoCarta_50640
Vesiclepedia VP_50640
Entrez Gene 50640
HGNC 28900
MIM 612123
UniProt Q9NP80  
 PNPLA8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PNPLA8
Molecular Function
    ATP binding GO:0005524 NAS
    lysophospholipase activity GO:0004622 IEA
    calcium-independent phospholipase A2 activity GO:0047499 IDA
Biological Process
    arachidonic acid metabolic process GO:0019369 IDA
    small molecule metabolic process GO:0044281 TAS
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    linoleic acid metabolic process GO:0043651 IDA
    phospholipid metabolic process GO:0006644 TAS
    arachidonic acid secretion GO:0050482 IDA
    cell death GO:0008219 IDA
    prostaglandin biosynthetic process GO:0001516 IDA
    phosphatidylcholine acyl-chain remodeling GO:0036151 TAS
    phosphatidylethanolamine catabolic process GO:0046338 IDA
    fatty acid metabolic process GO:0006631 IDA
    phosphatidylcholine catabolic process GO:0034638 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    peroxisomal membrane GO:0005778 IDA
    integral component of membrane GO:0016021 IEA
    peroxisome GO:0005777 IDA
    intracellular GO:0005622 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    Golgi membrane GO:0000139 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified PNPLA8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PNPLA8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PNPLA8 is involved
PathwayEvidenceSource
Acyl chain remodelling of PC TAS Reactome
Acyl chain remodelling of PE TAS Reactome





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