Gene description for PNPLA8
Gene name patatin-like phospholipase domain containing 8
Gene symbol PNPLA8
Other names/aliases IPLA2(GAMMA)
IPLA2-2
IPLA2G
MMLA
iPLA2gamma
Species Homo sapiens
 Database cross references - PNPLA8
ExoCarta ExoCarta_50640
Vesiclepedia VP_50640
Entrez Gene 50640
HGNC 28900
MIM 612123
UniProt Q9NP80  
 PNPLA8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PNPLA8
Molecular Function
    lysophospholipase activity GO:0004622 IEA
    ATP binding GO:0005524 NAS
    phospholipase A1 activity GO:0008970 IEA
    calcium-dependent phospholipase A2 activity GO:0047498 IEA
    calcium-independent phospholipase A2 activity GO:0047499 IBA
    calcium-independent phospholipase A2 activity GO:0047499 IDA
    calcium-independent phospholipase A2 activity GO:0047499 IMP
Biological Process
    prostaglandin biosynthetic process GO:0001516 IDA
    fatty acid metabolic process GO:0006631 IDA
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 IDA
    cardiolipin metabolic process GO:0032048 IDA
    phosphatidylcholine catabolic process GO:0034638 IDA
    intracellular signal transduction GO:0035556 IDA
    linoleic acid metabolic process GO:0043651 IDA
    phosphatidylethanolamine catabolic process GO:0046338 IDA
    arachidonate secretion GO:0050482 IDA
    lipid homeostasis GO:0055088 IMP
    triglyceride homeostasis GO:0070328 IMP
    regulation of cellular response to oxidative stress GO:1900407 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 ISS
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    mitochondrial membrane GO:0031966 IEA
 Experiment description of studies that identified PNPLA8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PNPLA8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 HNRNPD 3184
Affinity Capture-MS Homo sapiens
5 Pcbp2 18521
Affinity Capture-MS Mus musculus
6 C17orf80 55028
Proximity Label-MS Homo sapiens
7 PMPCB 9512
Proximity Label-MS Homo sapiens
8 TSFM 10102
Proximity Label-MS Homo sapiens
9 TBRG4 9238
Proximity Label-MS Homo sapiens
10 MTIF2 4528
Proximity Label-MS Homo sapiens
11 MDH2 4191
Proximity Label-MS Homo sapiens
12 MTIF3  
Proximity Label-MS Homo sapiens
13 LONP1 9361
Proximity Label-MS Homo sapiens
14 TRUB2  
Proximity Label-MS Homo sapiens
15 PDHA1 5160
Proximity Label-MS Homo sapiens
16 C6orf203  
Proximity Label-MS Homo sapiens
17 COX8A  
Proximity Label-MS Homo sapiens
18 C21orf33  
Proximity Label-MS Homo sapiens
19 DNAJC15  
Proximity Label-MS Homo sapiens
20 SIN3B  
Affinity Capture-MS Homo sapiens
21 SSBP1 6742
Proximity Label-MS Homo sapiens
22 CCDC90B  
Proximity Label-MS Homo sapiens
23 BAG6 7917
Affinity Capture-MS Homo sapiens
24 TUFM 7284
Proximity Label-MS Homo sapiens
25 SGTB  
Affinity Capture-MS Homo sapiens
26 GFM1 85476
Proximity Label-MS Homo sapiens
27 AARS2  
Proximity Label-MS Homo sapiens
28 PMPCA 23203
Proximity Label-MS Homo sapiens
29 S100P 6286
Affinity Capture-MS Homo sapiens
30 CCDC109B 55013
Proximity Label-MS Homo sapiens
31 CIT 11113
Affinity Capture-MS Homo sapiens
32 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
33 PLK4  
Affinity Capture-MS Homo sapiens
34 ACAD9 28976
Proximity Label-MS Homo sapiens
35 PRC1 9055
Affinity Capture-MS Homo sapiens
36 HINT2 84681
Proximity Label-MS Homo sapiens
37 KAT6A  
Affinity Capture-MS Homo sapiens
38 METTL17  
Proximity Label-MS Homo sapiens
39 AGPS 8540
Proximity Label-MS Homo sapiens
40 MRPS26 64949
Proximity Label-MS Homo sapiens
41 SURF1  
Proximity Label-MS Homo sapiens
42 TMEM70  
Proximity Label-MS Homo sapiens
43 PIPSL 266971
Affinity Capture-MS Homo sapiens
44 HYPM  
Affinity Capture-MS Homo sapiens
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