Experiment description of studies that identified HIST2H2AA4 in exosomes |
1 |
Experiment ID |
224 |
MISEV standards |
✔
EM|AFM
|
Biophysical techniques |
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
✔
GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
2 |
Experiment ID |
211 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
cytochrome c|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
3 |
Experiment ID |
212 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
Cytochrome C|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
138 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
5 |
Experiment ID |
139 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
6 |
Experiment ID |
141 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
7 |
Experiment ID |
142 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
8 |
Experiment ID |
143 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
9 |
Experiment ID |
144 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
C4-2 - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
10 |
Experiment ID |
145 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|RAB5|CD10
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
C4-2 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
11 |
Experiment ID |
146 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
HSP70|HSP90|CD9
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
"Hosseini-Beheshti E, Guns ES." |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
RWPE - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
12 |
Experiment ID |
66 |
MISEV standards |
✔
IEM
|
Biophysical techniques |
✔
TSG101|Alix|CD63|CD81
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
13 |
Experiment ID |
217 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|CD81|CD9|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|