Gene description for DHTKD1
Gene name dehydrogenase E1 and transketolase domain containing 1
Gene symbol DHTKD1
Other names/aliases AMOXAD
CMT2Q
Species Homo sapiens
 Database cross references - DHTKD1
ExoCarta ExoCarta_55526
Vesiclepedia VP_55526
Entrez Gene 55526
HGNC 23537
MIM 614984
UniProt Q96HY7  
 DHTKD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DHTKD1
Molecular Function
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IEA
    protein binding GO:0005515 IPI
    thiamine pyrophosphate binding GO:0030976 IEA
    2-oxoadipate dehydrogenase activity GO:0160166 IDA
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    generation of precursor metabolites and energy GO:0006091 IMP
    glycolytic process GO:0006096 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    oxoadipate dehydrogenase complex GO:0160167 IDA
 Experiment description of studies that identified DHTKD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHTKD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 DENR 8562
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
5 PMPCA 23203
Proximity Label-MS Homo sapiens
6 PMPCB 9512
Proximity Label-MS Homo sapiens
7 TSFM 10102
Proximity Label-MS Homo sapiens
8 AUH 549
Proximity Label-MS Homo sapiens
9 VWA8 23078
Proximity Label-MS Homo sapiens
10 CEP170P1  
Affinity Capture-MS Homo sapiens
11 NARS 4677
Co-fractionation Homo sapiens
12 DGKG  
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 ICAM1 3383
Affinity Capture-MS Homo sapiens
15 EML2 24139
Co-fractionation Homo sapiens
16 MTRF1L  
Proximity Label-MS Homo sapiens
17 MDH2 4191
Proximity Label-MS Homo sapiens
18 TRUB2  
Proximity Label-MS Homo sapiens
19 DLD 1738
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
20 IPO11 51194
Affinity Capture-MS Homo sapiens
21 MTG2  
Proximity Label-MS Homo sapiens
22 C6orf203  
Proximity Label-MS Homo sapiens
23 SSBP1 6742
Proximity Label-MS Homo sapiens
24 FAHD1 81889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 COX8A  
Proximity Label-MS Homo sapiens
26 MUT 4594
Co-fractionation Homo sapiens
27 MRRF  
Proximity Label-MS Homo sapiens
28 UNC45B  
Affinity Capture-MS Homo sapiens
29 C21orf33  
Proximity Label-MS Homo sapiens
30 ARNTL  
Two-hybrid Homo sapiens
31 RIMKLA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GATC  
Affinity Capture-MS Homo sapiens
33 Immt  
Affinity Capture-MS Mus musculus
34 C1QBP 708
Proximity Label-MS Homo sapiens
35 AARS2  
Proximity Label-MS Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 ANXA5 308
Co-fractionation Homo sapiens
38 ACAD9 28976
Proximity Label-MS Homo sapiens
39 ARNTL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 METTL17  
Proximity Label-MS Homo sapiens
41 MRPL11 65003
Proximity Label-MS Homo sapiens
42 PGLYRP3 114771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 YBEY  
Affinity Capture-MS Homo sapiens
44 MRPS26 64949
Proximity Label-MS Homo sapiens
45 MORN5  
Affinity Capture-MS Homo sapiens
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