Gene description for PDE4A
Gene name phosphodiesterase 4A, cAMP-specific
Gene symbol PDE4A
Other names/aliases DPDE2
PDE4
PDE46
Species Homo sapiens
 Database cross references - PDE4A
ExoCarta ExoCarta_5141
Vesiclepedia VP_5141
Entrez Gene 5141
HGNC 8780
MIM 600126
UniProt P27815  
 PDE4A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDE4A
Molecular Function
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 IBA
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 IDA
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 IGI
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 TAS
    protein binding GO:0005515 IPI
    cAMP binding GO:0030552 IGI
    metal ion binding GO:0046872 IEA
    3',5'-cyclic-GMP phosphodiesterase activity GO:0047555 IBA
Biological Process
    cAMP catabolic process GO:0006198 IDA
    cAMP catabolic process GO:0006198 IEA
    signal transduction GO:0007165 TAS
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    sensory perception of smell GO:0007608 IEA
    cAMP-mediated signaling GO:0019933 IBA
    cellular response to xenobiotic stimulus GO:0071466 IEA
    regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106070 IEA
    regulation of cAMP/PKA signal transduction GO:0141161 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 TAS
    extrinsic component of membrane GO:0019898 IDA
    ruffle membrane GO:0032587 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified PDE4A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDE4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Reconstituted Complex Homo sapiens
2 METAP2 10988
Affinity Capture-MS Homo sapiens
3 BTF3 689
Affinity Capture-MS Homo sapiens
4 ITPKB  
Affinity Capture-MS Homo sapiens
5 PRKAR2A 5576
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 AKAP6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 SQSTM1 8878
Affinity Capture-Western Homo sapiens
8 PDE4D  
Affinity Capture-MS Homo sapiens
9 NHLH2  
Affinity Capture-MS Homo sapiens
10 FKBP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PIAS4  
Biochemical Activity Homo sapiens
12 VEGFB  
Affinity Capture-MS Homo sapiens
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