ExoCarta: Gene summary

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Gene description for Stub1
Gene name	STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
Gene symbol	Stub1
Other names/aliases	Chip
Species	Rattus norvegicus
Database cross references - Stub1
ExoCarta	ExoCarta_287155
Entrez Gene	287155
Stub1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Stub1

Molecular Function
	Hsp70 protein binding	GO:0030544	ISO
	Hsp90 protein binding	GO:0051879	ISO
	ubiquitin-ubiquitin ligase activity	GO:0034450	ISO
	ubiquitin protein ligase activity	GO:0061630	ISO
	ubiquitin protein ligase binding	GO:0031625	ISO
	ligase activity	GO:0016874	IEA
	ubiquitin-protein transferase activity	GO:0004842	ISO
	misfolded protein binding	GO:0051787	ISO
	SMAD binding	GO:0046332	ISO
	enzyme binding	GO:0019899	ISO
	TPR domain binding	GO:0030911	ISO
	G-protein coupled receptor binding	GO:0001664	ISO
	kinase binding	GO:0019900	ISO
	protein binding	GO:0005515	IPI
	protein homodimerization activity	GO:0042803	ISO
Biological Process
	protein polyubiquitination	GO:0000209	ISO
	cellular response to misfolded protein	GO:0071218	ISO
	positive regulation of protein ubiquitination	GO:0031398	ISO
	positive regulation of ubiquitin-protein transferase activity	GO:0051443	ISO
	ubiquitin-dependent protein catabolic process	GO:0006511	ISO
	regulation of glucocorticoid metabolic process	GO:0031943	ISO
	misfolded or incompletely synthesized protein catabolic process	GO:0006515	ISO
	protein K63-linked ubiquitination	GO:0070534	ISO
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	ISO
	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	GO:0032436	ISO
	ubiquitin-dependent SMAD protein catabolic process	GO:0030579	ISO
	positive regulation of chaperone-mediated protein complex assembly	GO:0090035	ISO
	endoplasmic reticulum unfolded protein response	GO:0030968	ISO
	negative regulation of protein binding	GO:0032091	ISO
	protein autoubiquitination	GO:0051865	ISO
Subcellular Localization
	extracellular exosome	GO:0070062	ISO
	nucleoplasm	GO:0005654	ISO
	intermediate filament cytoskeleton	GO:0045111	ISO
	plasma membrane	GO:0005886	ISO
	cytoplasm	GO:0005737	ISO
	ubiquitin ligase complex	GO:0000151	ISO
	nuclear inclusion body	GO:0042405	ISO
	endoplasmic reticulum	GO:0005783	ISO

Experiment description of studies that identified Stub1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting