Gene description for Vdac1
Gene name voltage-dependent anion channel 1
Gene symbol Vdac1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vdac1
ExoCarta ExoCarta_83529
Vesiclepedia VP_83529
Entrez Gene 83529
UniProt Q9Z2L0  
 Vdac1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vdac1
Molecular Function
    voltage-gated monoatomic ion channel activity GO:0005244 ISO
    voltage-gated monoatomic ion channel activity GO:0005244 ISS
    monoatomic anion channel activity GO:0005253 IDA
    calcium channel activity GO:0005262 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    oxysterol binding GO:0008142 ISO
    oxysterol binding GO:0008142 ISS
    voltage-gated monoatomic anion channel activity GO:0008308 IBA
    voltage-gated monoatomic anion channel activity GO:0008308 IDA
    voltage-gated monoatomic anion channel activity GO:0008308 IEA
    voltage-gated monoatomic anion channel activity GO:0008308 ISO
    voltage-gated monoatomic anion channel activity GO:0008308 ISS
    porin activity GO:0015288 IEA
    cholesterol binding GO:0015485 ISO
    protein kinase binding GO:0019901 ISO
    voltage-gated channel activity GO:0022832 IDA
    phosphatidylcholine binding GO:0031210 ISO
    identical protein binding GO:0042802 ISO
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 IDA
    ceramide binding GO:0097001 ISO
Biological Process
    behavioral fear response GO:0001662 ISO
    monoatomic anion transport GO:0006820 ISO
    mitochondrial calcium ion transmembrane transport GO:0006851 IDA
    lipid transport GO:0006869 IEA
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    chemical synaptic transmission GO:0007268 ISO
    neuron-neuron synaptic transmission GO:0007270 ISO
    learning GO:0007612 ISO
    epithelial cell differentiation GO:0030855 ISO
    calcium import into the mitochondrion GO:0036444 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    monoatomic anion transmembrane transport GO:0098656 IEA
    negative regulation of calcium import into the mitochondrion GO:0110099 ISO
    regulation of mitophagy GO:1901524 ISO
    positive regulation of type 2 mitophagy GO:1905091 ISO
    mitochondrial transmembrane transport GO:1990542 ISO
    negative regulation of reactive oxygen species metabolic process GO:2000378 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane GO:0005741 TAS
    mitochondrial permeability transition pore complex GO:0005757 ISO
    mitochondrial permeability transition pore complex GO:0005757 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    synaptic vesicle GO:0008021 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    mitochondrial membrane GO:0031966 ISO
    protein-containing complex GO:0032991 IDA
    mitochondrial nucleoid GO:0042645 ISO
    membrane raft GO:0045121 IEA
    pore complex GO:0046930 IEA
    presynaptic active zone membrane GO:0048787 EXP
    presynaptic active zone membrane GO:0048787 IDA
    postsynaptic density membrane GO:0098839 IDA
 Experiment description of studies that identified Vdac1 in exosomes
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vdac1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BCL2L11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Uba52 64156
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 Stub1 287155
Affinity Capture-Western Rattus norvegicus
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