Gene description for Vcp
Gene name valosin-containing protein
Gene symbol Vcp
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vcp
ExoCarta ExoCarta_116643
Vesiclepedia VP_116643
Entrez Gene 116643
UniProt P46462  
 Vcp identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Pancreatic cancer cells 20124479    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Vcp
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 IMP
    ATP binding GO:0005524 ISO
    lipid binding GO:0008289 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IDA
    ATP hydrolysis activity GO:0016887 IMP
    ATP hydrolysis activity GO:0016887 ISO
    protein phosphatase binding GO:0019903 ISO
    protein domain specific binding GO:0019904 ISO
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    deubiquitinase activator activity GO:0035800 ISO
    K48-linked polyubiquitin modification-dependent protein binding GO:0036435 ISO
    MHC class I protein binding GO:0042288 ISO
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    ADP binding GO:0043531 IMP
    ADP binding GO:0043531 ISO
    ubiquitin-like protein ligase binding GO:0044389 ISO
    protein-containing complex binding GO:0044877 IPI
    cohesin loader activity GO:0061775 IEA
    ubiquitin-modified protein reader activity GO:0140036 ISO
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    BAT3 complex binding GO:1904288 ISO
    ubiquitin-specific protease binding GO:1990381 ISO
Biological Process
    DNA repair GO:0006281 ISS
    double-strand break repair GO:0006302 ISO
    double-strand break repair GO:0006302 ISS
    chromatin remodeling GO:0006338 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    NADH metabolic process GO:0006734 ISO
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISS
    proteasomal protein catabolic process GO:0010498 ISO
    proteasomal protein catabolic process GO:0010498 ISS
    positive regulation of mitochondrial membrane potential GO:0010918 ISO
    macroautophagy GO:0016236 ISO
    macroautophagy GO:0016236 ISS
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    viral genome replication GO:0019079 ISO
    translesion synthesis GO:0019985 ISO
    translesion synthesis GO:0019985 ISS
    retrograde protein transport, ER to cytosol GO:0030970 EXP
    retrograde protein transport, ER to cytosol GO:0030970 IBA
    retrograde protein transport, ER to cytosol GO:0030970 ISO
    positive regulation of protein-containing complex assembly GO:0031334 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 ISO
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 ISS
    cellular response to heat GO:0034605 ISO
    cellular response to heat GO:0034605 ISS
    negative regulation of hippo signaling GO:0035331 ISO
    stress granule disassembly GO:0035617 ISO
    stress granule disassembly GO:0035617 ISS
    interstrand cross-link repair GO:0036297 ISS
    ERAD pathway GO:0036503 IDA
    ERAD pathway GO:0036503 ISO
    ERAD pathway GO:0036503 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISS
    positive regulation of protein catabolic process GO:0045732 ISO
    negative regulation of smoothened signaling pathway GO:0045879 ISO
    ATP metabolic process GO:0046034 ISO
    regulation of synapse organization GO:0050807 IDA
    regulation of synapse organization GO:0050807 IMP
    mitotic spindle disassembly GO:0051228 IBA
    endoplasmic reticulum stress-induced pre-emptive quality control GO:0061857 ISO
    endoplasmic reticulum stress-induced pre-emptive quality control GO:0061857 ISS
    aggresome assembly GO:0070842 ISO
    cellular response to misfolded protein GO:0071218 ISO
    flavin adenine dinucleotide catabolic process GO:0072389 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    autophagosome maturation GO:0097352 IBA
    autophagosome maturation GO:0097352 ISO
    autophagosome maturation GO:0097352 ISS
    protein-DNA covalent cross-linking repair GO:0106300 ISO
    protein-DNA covalent cross-linking repair GO:0106300 ISS
    negative regulation of protein localization to chromatin GO:0120186 ISO
    cytoplasm protein quality control GO:0140455 ISO
    chromatin looping GO:0140588 IEA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    positive regulation of protein K63-linked deubiquitination GO:1903006 ISO
    positive regulation of Lys63-specific deubiquitinase activity GO:1903007 ISO
    regulation of aerobic respiration GO:1903715 ISO
    cellular response to arsenite ion GO:1903843 ISO
    cellular response to arsenite ion GO:1903843 ISS
    positive regulation of oxidative phosphorylation GO:1903862 ISO
    regulation of protein localization to chromatin GO:1905634 ISO
    regulation of protein localization to chromatin GO:1905634 ISS
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IMP
    positive regulation of ATP biosynthetic process GO:2001171 ISO
Subcellular Localization
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    protein-containing complex GO:0032991 ISO
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 EXP
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 IBA
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 IDA
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 ISO
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    Derlin-1 retrotranslocation complex GO:0036513 ISO
    synapse GO:0045202 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    ATPase complex GO:1904949 ISO
    VCP-NSFL1C complex GO:1990730 IPI
    VCP-NSFL1C complex GO:1990730 ISO
 Experiment description of studies that identified Vcp in exosomes
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
4
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
11
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Vcp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nf1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
2 Canx 29144
Co-fractionation Rattus norvegicus
3 Ubxn2b  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
4 Nsfl1c  
Affinity Capture-Western Rattus norvegicus
5 Cyp3a62  
Affinity Capture-Western Rattus norvegicus
6 Stub1 287155
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
7 Rad23b  
Affinity Capture-MS Rattus norvegicus
8 STXBP5L  
Affinity Capture-MS Homo sapiens
9 Ubxn2a  
Affinity Capture-Western Rattus norvegicus
10 Usp5 297593
Co-fractionation Rattus norvegicus
11 Ubc  
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
12 Vcp 116643
Co-fractionation Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Co-fractionation Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
13 Cacna1c  
Affinity Capture-Western Rattus norvegicus
14 Atxn3  
Affinity Capture-MS Mus musculus
15 Ubxn2a  
Affinity Capture-Western Mus musculus
16 Itpr1  
Affinity Capture-Western Rattus norvegicus
17 Clta 83800
Affinity Capture-Western Rattus norvegicus
18 Park7 117287
Affinity Capture-MS Rattus norvegicus
19 Chrna3  
Affinity Capture-Western Rattus norvegicus
20 B2m 24223
Co-fractionation Rattus norvegicus
21 Ufd1l  
Affinity Capture-Western Rattus norvegicus
22 Smad1  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml



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