Gene description for Usp5
Gene name ubiquitin specific peptidase 5 (isopeptidase T)
Gene symbol Usp5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Usp5
ExoCarta ExoCarta_297593
Vesiclepedia VP_297593
Entrez Gene 297593
 Usp5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Usp5
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    zinc ion binding GO:0008270 IEA
    ubiquitin binding GO:0043130 ISO
    deubiquitinase activity GO:0101005 ISO
Biological Process
    proteolysis GO:0006508 IEA
    regulation of protein stability GO:0031647 IBA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    protein K48-linked deubiquitination GO:0071108 ISO
    regulation protein catabolic process at presynapse GO:0140251 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified Usp5 in exosomes
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Usp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vcp 116643
Co-fractionation Rattus norvegicus
2 Rad23b  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Usp5 is involved
No pathways found





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