ExoCarta: Gene summary

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Gene description for Usp5
Gene name	ubiquitin specific peptidase 5 (isopeptidase T)
Gene symbol	Usp5
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Usp5
ExoCarta	ExoCarta_297593
Entrez Gene	297593
Usp5 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Usp5

Molecular Function
	ubiquitin-specific protease activity	GO:0004843	IBA
	omega peptidase activity	GO:0008242	IEA
	cysteine-type endopeptidase activity	GO:0004197	IBA
	zinc ion binding	GO:0008270	IEA
Biological Process
	protein K48-linked deubiquitination	GO:0071108	IEA
	regulation of proteasomal protein catabolic process	GO:0061136	IBA
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	IBA
	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	GO:0032436	IEA
	protein deubiquitination	GO:0016579	IBA
Subcellular Localization
	lysosome	GO:0005764	IBA

Experiment description of studies that identified Usp5 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting