Gene description for GSS
Gene name glutathione synthetase
Gene symbol GSS
Other names/aliases GSHS
HEL-S-64p
HEL-S-88n
Species Homo sapiens
 Database cross references - GSS
ExoCarta ExoCarta_2937
Entrez Gene 2937
HGNC 4624
MIM 601002
UniProt P48637  
 GSS identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for GSS
Molecular Function
    glycine binding GO:0016594 IEA
    ATP binding GO:0005524 IDA
    magnesium ion binding GO:0000287 IDA
    glutathione synthase activity GO:0004363 TAS
    protein homodimerization activity GO:0042803 IDA
    glutathione binding GO:0043295 IDA
Biological Process
    response to amino acid GO:0043200 IEA
    response to nutrient levels GO:0031667 IEA
    small molecule metabolic process GO:0044281 TAS
    aging GO:0007568 IEA
    glutathione biosynthetic process GO:0006750 TAS
    glutathione derivative biosynthetic process GO:1901687 TAS
    xenobiotic metabolic process GO:0006805 TAS
    response to cadmium ion GO:0046686 IEA
    cellular amino acid metabolic process GO:0006520 TAS
    response to tumor necrosis factor GO:0034612 IEA
    response to oxidative stress GO:0006979 TAS
    nervous system development GO:0007399 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GSS in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GSS
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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