Gene description for APOL1
Gene name
apolipoprotein L, 1
Gene symbol
APOL1
Other names/aliases
APO-L APOL APOL-I FSGS4
Species
Homo sapiens
Database cross references - APOL1
ExoCarta
ExoCarta_8542
Vesiclepedia
VP_8542
Entrez Gene
8542
HGNC
618
MIM
603743
UniProt
O14791
APOL1 identified in exosomes derived from the following tissue/cell type
Plasma
19028452
Plasma
36624553
Plasma
36624553
Platelets
25332113
Platelets
25332113
Platelets
25332113
Gene ontology annotations for APOL1
Experiment description of studies that identified APOL1 in exosomes
1
Experiment ID
44
MISEV standards
✔
IEM
EV Biophysical techniques
✘
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19028452
Organism
Homo sapiens
Experiment description
Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors
Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name
BBRC
Publication year
2009
Sample
Plasma
Sample name
Plasma
Isolation/purification methods
Filtration Size exclusion Sucrose density gradient
Flotation density
1.15-1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
2
Experiment ID
606
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
3
Experiment ID
607
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L.
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
4
Experiment ID
231
MISEV standards
✘
EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
5
Experiment ID
232
MISEV standards
✘
EV Biophysical techniques
✘
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
233
MISEV standards
✘
EV Biophysical techniques
✘
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for APOL1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
APOA1
335
Affinity Capture-MS
Homo sapiens
Co-purification
Homo sapiens
View the network
image/svg+xml
Pathways in which APOL1 is involved