Experiment description of studies that identified RASSF3 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for RASSF3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FAM46D   Two-hybrid Homo sapiens 2 STRIP1 85369 Affinity Capture-MS Homo sapiens 3 STK3 6788 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 BTRC 8945 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 FBXW11   Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 6 SLMAP 7871 Affinity Capture-MS Homo sapiens 7 VAPB 9217 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 STRN3 29966 Affinity Capture-MS Homo sapiens 9 VAPA 9218 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 SIKE1   Affinity Capture-MS Homo sapiens 11 XPO1 7514 Affinity Capture-MS Homo sapiens 12 TRIM59   Two-hybrid Homo sapiens 13 STRN4 29888 Affinity Capture-MS Homo sapiens 14 MAP1S 55201 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 CDC37 11140 Affinity Capture-MS Homo sapiens 16 LITAF 9516 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 17 MOB4 25843 Affinity Capture-MS Homo sapiens 18 ITPRIP   Two-hybrid Homo sapiens 19 MAP1B 4131 Affinity Capture-MS Homo sapiens 20 FAM110A   Two-hybrid Homo sapiens 21 APP 351 Reconstituted Complex Homo sapiens 22 STRN 6801 Affinity Capture-MS Homo sapiens 23 PARD6B 84612 Two-hybrid Homo sapiens 24 CBX8 57332 Two-hybrid Homo sapiens 25 ZNF410   Two-hybrid Homo sapiens 26 KYNU   Two-hybrid Homo sapiens 27 RPRD1B 58490 Two-hybrid Homo sapiens 28 STK4 6789 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 FGFR1OP2   Affinity Capture-MS Homo sapiens 30 MDM2   Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 31 DAXX   Two-hybrid Homo sapiens View the network

Pathways in which RASSF3 is involved

No pathways found