Gene ontology annotations for Hspa8 |
|
Experiment description of studies that identified Hspa8 in exosomes |
1 |
Experiment ID |
225 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25998041
|
Organism |
Rattus norvegicus |
Experiment description |
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. |
Authors |
Lee JE, Moon PG, Lee IK, Baek MC |
Journal name |
Protein J
|
Publication year |
2015 |
Sample |
Adipocytes |
Sample name |
Adipocytes |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
2 |
Experiment ID |
30 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
FLOT
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
16446100
|
Organism |
Rattus norvegicus |
Experiment description |
Exosomes are released by cultured cortical neurones. |
Authors |
Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R |
Journal name |
MCN
|
Publication year |
2006 |
Sample |
Cortical neurones |
Sample name |
Cortical neurones |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.13 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
3 |
Experiment ID |
35 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD81|CD63
|
EV Membrane markers |
✔
HSPA5
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19367702
|
Organism |
Rattus norvegicus |
Experiment description |
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. |
Authors |
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM |
Journal name |
JPR
|
Publication year |
2008 |
Sample |
Hepatocytes |
Sample name |
Hepatocytes |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
4 |
Experiment ID |
90 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSC70|HSP90|TSG101
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
5 |
Experiment ID |
94 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
6 |
Experiment ID |
95 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
7 |
Experiment ID |
96 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
8 |
Experiment ID |
97 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
9 |
Experiment ID |
98 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
10 |
Experiment ID |
99 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
11 |
Experiment ID |
100 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
12 |
Experiment ID |
101 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
✔
MHCI
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
Protein-protein interactions for Hspa8 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which Hspa8 is involved |
No pathways found
|
|
|