Gene description for Ap2b1
Gene name adaptor-related protein complex 2, beta 1 subunit
Gene symbol Ap2b1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ap2b1
ExoCarta ExoCarta_140670
Vesiclepedia VP_140670
Entrez Gene 140670
UniProt P62944  
 Ap2b1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ap2b1
Molecular Function
    protein binding GO:0005515 IPI
    clathrin binding GO:0030276 IBA
    clathrin binding GO:0030276 IDA
    clathrin binding GO:0030276 IEA
    clathrin binding GO:0030276 ISO
    protein-containing complex binding GO:0044877 IPI
Biological Process
    kidney development GO:0001822 ISO
    cardiac septum development GO:0003279 ISO
    ventricular septum development GO:0003281 ISO
    intracellular protein transport GO:0006886 IEA
    heart development GO:0007507 ISO
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IEA
    aorta development GO:0035904 ISO
    positive regulation of endocytosis GO:0045807 IMP
    clathrin coat assembly GO:0048268 IDA
    synaptic vesicle endocytosis GO:0048488 ISO
    coronary vasculature development GO:0060976 ISO
    clathrin-dependent endocytosis GO:0072583 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 EXP
    postsynaptic neurotransmitter receptor internalization GO:0098884 IDA
    postsynaptic neurotransmitter receptor internalization GO:0098884 IMP
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
    positive regulation of protein localization to membrane GO:1905477 IMP
Subcellular Localization
    synaptic vesicle GO:0008021 EXP
    synaptic vesicle GO:0008021 IDA
    endomembrane system GO:0012505 IEA
    clathrin coat GO:0030118 IDA
    AP-type membrane coat adaptor complex GO:0030119 TAS
    AP-2 adaptor complex GO:0030122 ISO
    clathrin adaptor complex GO:0030131 IEA
    clathrin adaptor complex GO:0030131 ISO
    cytoplasmic vesicle GO:0031410 IEA
    synapse GO:0045202 ISO
    postsynaptic endocytic zone GO:0098843 IDA
    postsynaptic endocytic zone GO:0098843 IMP
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 IDA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 NAS
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    glutamatergic synapse GO:0098978 NAS
 Experiment description of studies that identified Ap2b1 in exosomes
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ap2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epn1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
2 Tfrc 64678
Reconstituted Complex Rattus norvegicus
3 Arrb2  
Affinity Capture-Western Rattus norvegicus
4 Eps15 313474
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
5 Itsn2  
Affinity Capture-MS Rattus norvegicus
6 Bin1  
Affinity Capture-Western Rattus norvegicus
7 MTNR1B  
Two-hybrid Homo sapiens
8 Arfgap1  
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
9 Scyl1  
Affinity Capture-MS Rattus norvegicus
10 Amph  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
11 Mybl2  
Affinity Capture-MS Rattus norvegicus
12 Snap91  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
13 Dnm1 140694
Affinity Capture-MS Rattus norvegicus
14 Rangap1 362965
Affinity Capture-MS Rattus norvegicus
15 Dao  
Affinity Capture-MS Rattus norvegicus
16 Hspa8 24468
Affinity Capture-MS Rattus norvegicus
17 Itsn1  
Affinity Capture-MS Rattus norvegicus
18 Krt1 300250
Affinity Capture-MS Rattus norvegicus
19 Cltc 54241
Affinity Capture-MS Rattus norvegicus
20 HIP1 3092
Two-hybrid Homo sapiens
21 Synrg  
Affinity Capture-MS Rattus norvegicus
22 Tollip 361677
Affinity Capture-MS Rattus norvegicus
23 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
24 Tom1 361370
Affinity Capture-MS Rattus norvegicus
25 Epn2  
Affinity Capture-MS Rattus norvegicus
26 Dab2 13132
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Ap2b1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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