ExoCarta: Gene summary

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Gene description for Eps15
Gene name	epidermal growth factor receptor pathway substrate 15
Gene symbol	Eps15
Other names/aliases	RGD1307641
Species	Rattus norvegicus
Database cross references - Eps15
ExoCarta	ExoCarta_313474
Entrez Gene	313474
Eps15 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Eps15

Molecular Function
	calcium ion binding	GO:0005509	IEA
	polyubiquitin binding	GO:0031593	ISO
	identical protein binding	GO:0042802	ISO
	protein binding	GO:0005515	IPI
Biological Process
	Golgi to endosome transport	GO:0006895	ISO
	clathrin coat assembly	GO:0048268	ISO
	receptor-mediated endocytosis of virus by host cell	GO:0019065	ISO
	viral entry into host cell	GO:0046718	ISO
	positive regulation of receptor recycling	GO:0001921	IMP
	regulation of cell proliferation	GO:0042127	IMP
	endocytic recycling	GO:0032456	IMP
Subcellular Localization
	plasma membrane	GO:0005886	ISO
	clathrin-coated vesicle	GO:0030136	ISO
	ciliary membrane	GO:0060170	ISO
	cytosol	GO:0005829	IDA
	intracellular membrane-bounded organelle	GO:0043231	ISO
	early endosome	GO:0005769	IDA
	basal plasma membrane	GO:0009925	IDA
	coated pit	GO:0005905	ISO
	clathrin coat of coated pit	GO:0030132	ISO
	apical plasma membrane	GO:0016324	IDA
	membrane	GO:0016020	ISO
	aggresome	GO:0016235	ISO
	cytoplasm	GO:0005737	ISO
	AP-2 adaptor complex	GO:0030122	ISO

Experiment description of studies that identified Eps15 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting