Gene description for Eps15
Gene name epidermal growth factor receptor pathway substrate 15
Gene symbol Eps15
Other names/aliases RGD1307641
Species Rattus norvegicus
 Database cross references - Eps15
ExoCarta ExoCarta_313474
Vesiclepedia VP_313474
Entrez Gene 313474
 Eps15 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eps15
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    identical protein binding GO:0042802 ISO
    ubiquitin binding GO:0043130 ISO
Biological Process
    positive regulation of receptor recycling GO:0001921 IMP
    Golgi to endosome transport GO:0006895 ISO
    endocytosis GO:0006897 IBA
    endosomal transport GO:0016197 IBA
    receptor-mediated endocytosis of virus by host cell GO:0019065 ISO
    endocytic recycling GO:0032456 IMP
    regulation of protein localization GO:0032880 ISO
    regulation of cell population proliferation GO:0042127 IMP
    symbiont entry into host cell GO:0046718 ISO
    clathrin coat assembly GO:0048268 ISO
    ubiquitin-dependent endocytosis GO:0070086 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 IDA
    postsynaptic neurotransmitter receptor internalization GO:0098884 IEP
    postsynaptic neurotransmitter receptor internalization GO:0098884 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 ISO
    basal plasma membrane GO:0009925 IDA
    aggresome GO:0016235 ISO
    apical plasma membrane GO:0016324 IDA
    AP-2 adaptor complex GO:0030122 ISO
    clathrin coat of coated pit GO:0030132 IBA
    clathrin coat of coated pit GO:0030132 ISO
    clathrin-coated vesicle GO:0030136 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    synapse GO:0045202 IDA
    ciliary membrane GO:0060170 ISO
    postsynaptic endocytic zone GO:0098843 IDA
    postsynaptic endocytic zone GO:0098843 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Eps15 in exosomes
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eps15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fcho2  
Reconstituted Complex Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
2 Nme2 83782
Affinity Capture-Western Rattus norvegicus
3 SGIP1  
Reconstituted Complex Homo sapiens
4 Ap2a1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
5 FCHO1  
Reconstituted Complex Homo sapiens
6 Ap2a2  
Affinity Capture-Western Rattus norvegicus
7 Gria1  
Affinity Capture-Western Rattus norvegicus
8 Necap2  
Affinity Capture-Western Rattus norvegicus
9 Synj1 85238
Reconstituted Complex Rattus norvegicus
10 Necap1  
Affinity Capture-Western Rattus norvegicus
11 Ap2a2 11772
Reconstituted Complex Mus musculus
12 PARK2  
Reconstituted Complex Homo sapiens
13 Slc6a3  
Affinity Capture-Western Rattus norvegicus
14 Mob4  
Two-hybrid Rattus norvegicus
15 Ubc  
Affinity Capture-Western Rattus norvegicus
16 Ap2b1 140670
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Eps15 is involved
No pathways found





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