Gene description for Synj1
Gene name synaptojanin 1
Gene symbol Synj1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Synj1
ExoCarta ExoCarta_85238
Vesiclepedia VP_85238
Entrez Gene 85238
UniProt Q62910  
 Synj1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Synj1
Molecular Function
    RNA binding GO:0003723 IEA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IEA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 ISO
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IBA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IDA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IEA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 ISO
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IDA
    SH3 domain binding GO:0017124 IPI
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 IDA
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 ISO
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 IEA
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 ISO
    protein-containing complex binding GO:0044877 IDA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IBA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IDA
    inositol phosphate phosphatase activity GO:0052745 IEA
    phosphatidylinositol phosphate phosphatase activity GO:0052866 IEA
    EH domain binding GO:1990175 IDA
Biological Process
    neurotransmitter transport GO:0006836 ISO
    learning GO:0007612 IEA
    learning GO:0007612 ISO
    learning GO:0007612 ISO
    positive regulation of gliogenesis GO:0014015 IMP
    synaptic vesicle priming GO:0016082 ISO
    synaptic vesicle uncoating GO:0016191 ISO
    synaptic vesicle uncoating GO:0016191 ISO
    response to retinoic acid GO:0032526 IMP
    response to cytokine GO:0034097 IMP
    phosphatidylinositol metabolic process GO:0046488 ISO
    phosphatidylinositol dephosphorylation GO:0046856 IEA
    phosphatidylinositol dephosphorylation GO:0046856 ISO
    phosphatidylinositol dephosphorylation GO:0046856 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 IMP
    synaptic vesicle endocytosis GO:0048488 IBA
    synaptic vesicle endocytosis GO:0048488 IEA
    synaptic vesicle endocytosis GO:0048488 ISO
    synaptic vesicle endocytosis GO:0048488 ISO
    synaptic vesicle endocytosis GO:0048488 ISS
    synaptic vesicle transport GO:0048489 ISO
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 ISO
    positive regulation of endosome organization GO:1904980 IEA
    positive regulation of endosome organization GO:1904980 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    microtubule GO:0005874 IDA
    vesicle membrane GO:0012506 ISS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    membrane coat GO:0030117 ISO
    clathrin coat of coated pit GO:0030132 IDA
    protein-containing complex GO:0032991 IDA
    terminal bouton GO:0043195 ISS
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 ISS
    synaptic membrane GO:0097060 IDA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 IDA
    presynapse GO:0098793 IBA
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Synj1 in exosomes
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Synj1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fcho2  
Affinity Capture-MS Mus musculus
2 Sorbs2  
Reconstituted Complex Rattus norvegicus
3 Ppp3ca  
Biochemical Activity Rattus norvegicus
4 EPS15 2060
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
5 Ap2a2  
Affinity Capture-Western Rattus norvegicus
6 Eps15 313474
Reconstituted Complex Rattus norvegicus
7 Amph  
Affinity Capture-Western Rattus norvegicus
8 GRB2 2885
Reconstituted Complex Homo sapiens
9 Necap1  
Affinity Capture-Western Rattus norvegicus
10 Sh3gl2  
Affinity Capture-Western Rattus norvegicus
11 Necap2  
Affinity Capture-Western Rattus norvegicus
12 Bin1  
Affinity Capture-Western Rattus norvegicus
13 Cdk5  
Biochemical Activity Rattus norvegicus
14 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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