Gene description for MT1E
Gene name metallothionein 1E
Gene symbol MT1E
Other names/aliases MT-1E
MT-IE
MT1
MTD
Species Homo sapiens
 Database cross references - MT1E
ExoCarta ExoCarta_4493
Vesiclepedia VP_4493
Entrez Gene 4493
HGNC 7397
MIM 156351
UniProt P04732  
 MT1E identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MT1E
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISS
    metal ion binding GO:0046872 IBA
Biological Process
    intracellular zinc ion homeostasis GO:0006882 IBA
    detoxification of copper ion GO:0010273 IBA
    negative regulation of growth GO:0045926 ISS
    cellular response to cadmium ion GO:0071276 IBA
    cellular response to cadmium ion GO:0071276 IEP
    cellular response to copper ion GO:0071280 IBA
    cellular response to zinc ion GO:0071294 IBA
    cellular response to zinc ion GO:0071294 IEP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified MT1E in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MT1E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK2 1017
Affinity Capture-MS Homo sapiens
2 RNF181  
Two-hybrid Homo sapiens
3 TRIM37  
Proximity Label-MS Homo sapiens
4 CCDC57 284001
Two-hybrid Homo sapiens
5 MT1M 4499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MT1E is involved
PathwayEvidenceSource
Cellular responses to stimuli IEA Reactome
Metallothioneins bind metals IEA Reactome
Response to metal ions IEA Reactome





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