Gene description for Wipi2
Gene name WD repeat domain, phosphoinositide interacting 2
Gene symbol Wipi2
Other names/aliases 1110018O08Rik
2510001I10Rik
Species Mus musculus
 Database cross references - Wipi2
ExoCarta ExoCarta_74781
Vesiclepedia VP_74781
Entrez Gene 74781
UniProt Q80W47  
 Wipi2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wipi2
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-5-phosphate binding GO:0010314 ISO
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 ISO
    phosphatidylinositol-3-phosphate binding GO:0032266 ISS
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISO
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISS
Biological Process
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    autophagy of mitochondrion GO:0000422 IBA
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    autophagy of peroxisome GO:0030242 IBA
    protein localization to phagophore assembly site GO:0034497 IBA
    protein localization to phagophore assembly site GO:0034497 IPI
    protein localization to phagophore assembly site GO:0034497 ISO
    nucleophagy GO:0044804 IBA
    glycophagy GO:0061723 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IDA
    xenophagy GO:0098792 IPI
Subcellular Localization
    phagophore assembly site GO:0000407 IDA
    phagophore assembly site GO:0000407 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    autophagosome GO:0005776 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    membrane GO:0016020 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 ISO
    phagophore assembly site membrane GO:0034045 ISS
 Experiment description of studies that identified Wipi2 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wipi2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nudc 18221
Co-fractionation Mus musculus
2 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
3 PACS2 23241
Affinity Capture-MS Homo sapiens
4 Tmem173  
Proximity Label-MS Mus musculus
5 GTF2F1 2962
Affinity Capture-MS Homo sapiens
6 PLAUR 5329
Affinity Capture-MS Homo sapiens
7 BCAS3 54828
Affinity Capture-MS Homo sapiens
8 DIS3L2 129563
Affinity Capture-MS Homo sapiens
9 DSG1 1828
Affinity Capture-MS Homo sapiens
10 Atg12  
Positive Genetic Mus musculus
11 MYO10 4651
Affinity Capture-MS Homo sapiens
12 Aip  
Co-fractionation Mus musculus
13 Dnm2 13430
Co-fractionation Mus musculus
14 MACF1 23499
Affinity Capture-MS Homo sapiens
15 CHD9 80205
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Wipi2 is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Macroautophagy IEA Reactome





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