Gene description for Wipi2
Gene name WD repeat domain, phosphoinositide interacting 2
Gene symbol Wipi2
Other names/aliases 1110018O08Rik
2510001I10Rik
Species Mus musculus
 Database cross references - Wipi2
ExoCarta ExoCarta_74781
Entrez Gene 74781
UniProt Q80W47  
 Wipi2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wipi2
Molecular Function
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISO
    phosphatidylinositol-3-phosphate binding GO:0032266 ISO
Biological Process
    protein lipidation GO:0006497 IBA
    mitochondrion degradation GO:0000422 IBA
    protein localization to pre-autophagosomal structure GO:0034497 IBA
    autophagy GO:0006914 IEA
    nucleophagy GO:0044804 IBA
    autophagosome assembly GO:0000045 ISO
    cellular response to nitrogen starvation GO:0006995 IBA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    protein complex GO:0043234 ISO
    pre-autophagosomal structure membrane GO:0034045 ISO
    pre-autophagosomal structure GO:0000407 ISO
    membrane GO:0016020 IEA
    cytoplasm GO:0005737 ISO
    extrinsic component of membrane GO:0019898 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Wipi2 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wipi2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wipi2 is involved
No pathways found





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