Gene description for SIGIRR
Gene name single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
Gene symbol SIGIRR
Other names/aliases TIR8
Species Homo sapiens
 Database cross references - SIGIRR
ExoCarta ExoCarta_59307
Vesiclepedia VP_59307
Entrez Gene 59307
HGNC 30575
MIM 605478
UniProt Q6IA17  
 SIGIRR identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for SIGIRR
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of chemokine biosynthetic process GO:0045079 ISS
    acute-phase response GO:0006953 ISS
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IMP
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 TAS
    negative regulation of cytokine-mediated signaling pathway GO:0001960 ISS
    signal transduction GO:0007165 IEA
Subcellular Localization
    membrane GO:0016020 IMP
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified SIGIRR in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SIGIRR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENO2 2026
Two-hybrid Homo sapiens
2 TLR4  
Affinity Capture-Western Homo sapiens
3 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 IRAK1  
Affinity Capture-Western Homo sapiens
5 SIGIRR 59307
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 IL1R1 3554
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SIGIRR is involved
PathwayEvidenceSource
MyD88:Mal cascade initiated on plasma membrane TAS Reactome





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