Gene description for Smc4
Gene name structural maintenance of chromosomes 4
Gene symbol Smc4
Other names/aliases 2500002A22Rik
C79747
SMC-4
Smc4l1
Species Mus musculus
 Database cross references - Smc4
ExoCarta ExoCarta_70099
Vesiclepedia VP_70099
Entrez Gene 70099
UniProt Q8CG47  
 Smc4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smc4
Molecular Function
    chromatin binding GO:0003682 IDA
    single-stranded DNA binding GO:0003697 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    single strand break repair GO:0000012 TAS
    mitotic chromosome condensation GO:0007076 ISO
    mitotic chromosome condensation GO:0007076 ISS
    meiotic chromosome condensation GO:0010032 IMP
    meiotic chromosome segregation GO:0045132 IMP
    cell division GO:0051301 IEA
    kinetochore organization GO:0051383 IMP
    positive regulation of chromosome segregation GO:0051984 IMP
    positive regulation of chromosome separation GO:1905820 IMP
    positive regulation of chromosome condensation GO:1905821 ISO
    positive regulation of chromosome condensation GO:1905821 ISS
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    condensed nuclear chromosome GO:0000794 IDA
    condensin complex GO:0000796 IDA
    condensin complex GO:0000796 ISO
    condensin complex GO:0000796 NAS
    condensin complex GO:0000796 TAS
    nucleus GO:0005634 TAS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
 Experiment description of studies that identified Smc4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smc4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCAPH2  
Affinity Capture-MS Homo sapiens
2 NCAPD2 9918
Affinity Capture-MS Homo sapiens
3 NCAPG 64151
Affinity Capture-MS Homo sapiens
4 Smarca5 93762
Co-fractionation Mus musculus
5 Plk4  
Co-fractionation Mus musculus
6 NCAPG2 54892
Affinity Capture-MS Homo sapiens
7 Eed  
Affinity Capture-MS Mus musculus
8 NCAPH 23397
Affinity Capture-MS Homo sapiens
9 SMC2 10592
Affinity Capture-MS Homo sapiens
10 Ncapd2 68298
Co-fractionation Mus musculus
11 NCAPD3 23310
Affinity Capture-MS Homo sapiens
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 Kif4  
Co-fractionation Mus musculus
14 Zwilch  
Co-fractionation Mus musculus
15 Ncapg2  
Co-fractionation Mus musculus
16 Aspm  
Co-fractionation Mus musculus
17 Fbxo5  
Co-fractionation Mus musculus
18 Smc2 14211
Co-fractionation Mus musculus
19 Ncaph  
Co-fractionation Mus musculus
20 Ncapd3  
Co-fractionation Mus musculus
21 Kif23 71819
Co-fractionation Mus musculus
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