Gene description for Smc2
Gene name structural maintenance of chromosomes 2
Gene symbol Smc2
Other names/aliases 5730502P04Rik
AI255214
AW545314
CAP-E
CAPE
Fin16
SMC-2
Smc2l1
Species Mus musculus
 Database cross references - Smc2
ExoCarta ExoCarta_14211
Vesiclepedia VP_14211
Entrez Gene 14211
UniProt Q8CG48  
 Smc2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smc2
Molecular Function
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    single-stranded DNA binding GO:0003697 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    single strand break repair GO:0000012 TAS
    mitotic chromosome condensation GO:0007076 IBA
    mitotic chromosome condensation GO:0007076 IEA
    mitotic chromosome condensation GO:0007076 ISO
    meiotic chromosome condensation GO:0010032 IMP
    meiotic chromosome segregation GO:0045132 IMP
    cell division GO:0051301 IEA
    kinetochore organization GO:0051383 IMP
    positive regulation of chromosome segregation GO:0051984 IMP
    positive regulation of chromosome separation GO:1905820 IMP
    positive regulation of chromosome condensation GO:1905821 ISO
    positive regulation of chromosome condensation GO:1905821 ISS
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    chromatin GO:0000785 IBA
    condensed chromosome GO:0000793 IBA
    condensed chromosome GO:0000793 IDA
    condensed chromosome GO:0000793 ISO
    condensed nuclear chromosome GO:0000794 IDA
    condensin complex GO:0000796 IBA
    condensin complex GO:0000796 IDA
    condensin complex GO:0000796 IPI
    condensin complex GO:0000796 ISO
    condensin complex GO:0000796 NAS
    condensin complex GO:0000796 TAS
    nucleus GO:0005634 ISO
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Smc2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smc2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Creb3l4  
Affinity Capture-MS Mus musculus
2 Fbxo5  
Co-fractionation Mus musculus
3 Cenpk  
Co-fractionation Mus musculus
4 Sin3a  
Co-fractionation Mus musculus
5 Tsc1  
Affinity Capture-MS Mus musculus
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Pbk  
Co-fractionation Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Ncapg2  
Co-fractionation Mus musculus
10 Arhgap11a  
Co-fractionation Mus musculus
11 Ncaph  
Co-fractionation Mus musculus
12 Crem  
Affinity Capture-MS Mus musculus
13 Plk4  
Co-fractionation Mus musculus
14 Kif4  
Co-fractionation Mus musculus
15 Mki67 17345
Co-fractionation Mus musculus
16 Ncapd3  
Co-fractionation Mus musculus
17 Smarca5 93762
Co-fractionation Mus musculus
18 Eed  
Affinity Capture-MS Mus musculus
19 Ncapd2 68298
Co-fractionation Mus musculus
20 Smc4 70099
Co-fractionation Mus musculus
21 Cenpe  
Co-fractionation Mus musculus
22 Fancd2  
Affinity Capture-MS Mus musculus
23 Kif23 71819
Co-fractionation Mus musculus
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