Gene description for Smarca5
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Gene symbol Smarca5
Other names/aliases 4933427E24Rik
D030040M08Rik
D330027N15Rik
MommeD4
Snf2h
Species Mus musculus
 Database cross references - Smarca5
ExoCarta ExoCarta_93762
Vesiclepedia VP_93762
Entrez Gene 93762
UniProt Q91ZW3  
 Smarca5 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smarca5
Molecular Function
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IEA
    nucleotide binding GO:0000166 IEA
    hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides GO:0016818 IEA
    histone binding GO:0042393 ISO
    hydrolase activity GO:0016787 IEA
    hydrolase activity, acting on acid anhydrides GO:0016817 IEA
    nucleosome binding GO:0031491 IEA
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISO
    ATPase activity GO:0016887 ISO
    helicase activity GO:0004386 IEA
Biological Process
    nucleosome positioning GO:0016584 ISO
    nucleosome assembly GO:0006334 ISO
    chromatin remodeling GO:0006338 ISO
    chromatin assembly or disassembly GO:0006333 IDA
    double-strand break repair GO:0006302 IMP
    chromatin modification GO:0016568 IEA
    positive regulation of transcription, DNA-templated GO:0045893 TAS
    ATP-dependent chromatin remodeling GO:0043044 ISO
    DNA-templated transcription, initiation GO:0006352 ISO
    embryo development GO:0009790 IMP
    chromatin silencing at rDNA GO:0000183 IDA
Subcellular Localization
    RSF complex GO:0031213 ISO
    condensed chromosome GO:0000793 ISO
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    ISWI-type complex GO:0031010 IPI
    NURF complex GO:0016589 ISO
    chromatin silencing complex GO:0005677 IDA
    nuclear replication fork GO:0043596 ISO
 Experiment description of studies that identified Smarca5 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smarca5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Baz2a  
Co-fractionation Mus musculus
Affinity Capture-MS Mus musculus
2 Baz1b 22385
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 BC065123  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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