Gene description for C6
Gene name complement component 6
Gene symbol C6
Other names/aliases -
Species Homo sapiens
 Database cross references - C6
ExoCarta ExoCarta_729
Vesiclepedia VP_729
Entrez Gene 729
HGNC 1339
MIM 217050
UniProt P13671  
 C6 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C6
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    in utero embryonic development GO:0001701 IEA
    complement activation GO:0006956 IBA
    complement activation GO:0006956 IDA
    complement activation, classical pathway GO:0006958 IEA
    killing of cells of another organism GO:0031640 IEA
    innate immune response GO:0045087 IEA
    positive regulation of immune response GO:0050778 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    membrane attack complex GO:0005579 IBA
    membrane attack complex GO:0005579 IDA
    membrane attack complex GO:0005579 IPI
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified C6 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SART3 9733
Two-hybrid Homo sapiens
2 C5 727
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 DPY19L3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NPC2 10577
Affinity Capture-MS Homo sapiens
5 SPTBN2 6712
Cross-Linking-MS (XL-MS) Homo sapiens
6 CP 1356
Cross-Linking-MS (XL-MS) Homo sapiens
7 FUS 2521
Affinity Capture-MS Homo sapiens
8 APOA1 335
Cross-Linking-MS (XL-MS) Homo sapiens
9 ACOT9 23597
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 B3GALTL 145173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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