Gene description for SPTBN5
Gene name spectrin, beta, non-erythrocytic 5
Gene symbol SPTBN5
Other names/aliases BSPECV
HUBSPECV
HUSPECV
Species Homo sapiens
 Database cross references - SPTBN5
ExoCarta ExoCarta_51332
Vesiclepedia VP_51332
Entrez Gene 51332
HGNC 15680
MIM 605916
UniProt Q9NRC6  
 SPTBN5 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SPTBN5
Molecular Function
    opsin binding GO:0002046 IEA
    actin binding GO:0003779 NAS
    kinesin binding GO:0019894 IDA
    spectrin binding GO:0030507 IDA
    myosin tail binding GO:0032029 IDA
    dynactin binding GO:0034452 IDA
    identical protein binding GO:0042802 IDA
    dynein intermediate chain binding GO:0045505 IDA
    actin filament binding GO:0051015 IBA
Biological Process
    Golgi organization GO:0007030 IMP
    lysosomal transport GO:0007041 IMP
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 NAS
    actin filament capping GO:0051693 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    microtubule associated complex GO:0005875 IEA
    plasma membrane GO:0005886 IBA
    spectrin GO:0008091 NAS
    membrane GO:0016020 NAS
    cell junction GO:0030054 IBA
    cortical actin cytoskeleton GO:0030864 IBA
    photoreceptor connecting cilium GO:0032391 IDA
    cell projection GO:0042995 IBA
    apical cortex GO:0045179 IEA
    photoreceptor disc membrane GO:0097381 IDA
 Experiment description of studies that identified SPTBN5 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SPTBN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 CUL7 9820
Affinity Capture-MS Homo sapiens
3 GAN 8139
Affinity Capture-MS Homo sapiens
4 SPTAN1 6709
Co-fractionation Homo sapiens
5 MYC  
Affinity Capture-MS Homo sapiens
6 RHOT2 89941
Proximity Label-MS Homo sapiens
7 PARK2  
Affinity Capture-MS Homo sapiens
8 LMAN1 3998
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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