Gene description for SPTBN5
Gene name spectrin, beta, non-erythrocytic 5
Gene symbol SPTBN5
Other names/aliases BSPECV
HUBSPECV
HUSPECV
Species Homo sapiens
 Database cross references - SPTBN5
ExoCarta ExoCarta_51332
Entrez Gene 51332
HGNC 15680
MIM 605916
UniProt Q9NRC6  
 SPTBN5 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for SPTBN5
Molecular Function
    dynein intermediate chain binding GO:0045505 IDA
    kinesin binding GO:0019894 IDA
    spectrin binding GO:0030507 IDA
    actin binding GO:0003779 NAS
    myosin tail binding GO:0032029 IDA
    dynactin binding GO:0034452 IDA
    protein self-association GO:0043621 IDA
Biological Process
    Golgi organization GO:0007030 IMP
    lysosomal transport GO:0007041 IMP
    axon guidance GO:0007411 TAS
    protein homooligomerization GO:0051260 IDA
    actin filament capping GO:0051693 IEA
    actin cytoskeleton organization GO:0030036 NAS
Subcellular Localization
    membrane GO:0016020 NAS
    cytoplasm GO:0005737 IDA
    protein complex GO:0043234 IEA
    photoreceptor disc membrane GO:0097381 IDA
    spectrin GO:0008091 NAS
    photoreceptor connecting cilium GO:0032391 IDA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SPTBN5 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SPTBN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPTBN5 is involved
PathwayEvidenceSource
Interaction between L1 and Ankyrins TAS Reactome
NCAM signaling for neurite out-growth TAS Reactome





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