Gene description for DEAF1
Gene name DEAF1 transcription factor
Gene symbol DEAF1
Other names/aliases MRD24
NUDR
SPN
ZMYND5
Species Homo sapiens
 Database cross references - DEAF1
ExoCarta ExoCarta_10522
Entrez Gene 10522
HGNC 14677
MIM 602635
UniProt O75398  
 DEAF1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DEAF1
Molecular Function
    metal ion binding GO:0046872 IEA
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    neural tube closure GO:0001843 ISS
    regulation of mammary gland epithelial cell proliferation GO:0033599 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    transcription from RNA polymerase II promoter GO:0006366 IEA
    germ cell development GO:0007281 TAS
    visual learning GO:0008542 IEA
    anatomical structure morphogenesis GO:0009653 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
    embryonic skeletal system development GO:0048706 ISS
    behavioral fear response GO:0001662 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    extracellular region GO:0005576 IEA
    transcription factor complex GO:0005667 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified DEAF1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DEAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASCC2  
Two-hybrid Homo sapiens
2 LMO4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DEAF1 is involved
No pathways found





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