 Experiment description of studies that identified HIST1H2AL in sEVs |
| 1 |
| Experiment ID |
226 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|CD9|FLOT1
|
Enriched markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" |
| Authors |
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 2 |
| Experiment ID |
236 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
MHCC97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 3 |
| Experiment ID |
237 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocytes |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA Sequencing |
|
|
| 4 |
| Experiment ID |
224 |
| MISEV standards |
|
✔
EM|AFM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 5 |
| Experiment ID |
138 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 6 |
| Experiment ID |
140 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 7 |
| Experiment ID |
141 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 8 |
| Experiment ID |
142 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 9 |
| Experiment ID |
144 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 10 |
| Experiment ID |
145 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD10
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 11 |
| Experiment ID |
146 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90|CD9
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
"Hosseini-Beheshti E, Guns ES." |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
RWPE - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
