Gene description for RNASE4
Gene name ribonuclease, RNase A family, 4
Gene symbol RNASE4
Other names/aliases RAB1
RNS4
Species Homo sapiens
 Database cross references - RNASE4
ExoCarta ExoCarta_6038
Entrez Gene 6038
HGNC 10047
MIM 601030
UniProt P34096  
 RNASE4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
 Gene ontology annotations for RNASE4
Molecular Function
    ribonuclease A activity GO:0004522 NAS
    nucleic acid binding GO:0003676 IEA
Biological Process
    cellular response to starvation GO:0009267 IEA
    mRNA cleavage GO:0006379 NAS
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 NAS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified RNASE4 in exosomes
1
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RNASE4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNASE4 6038
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RNASE4 is involved
No pathways found





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