Gene description for MBTPS1
Gene name membrane-bound transcription factor peptidase, site 1
Gene symbol MBTPS1
Other names/aliases PCSK8
S1P
SKI-1
Species Homo sapiens
 Database cross references - MBTPS1
ExoCarta ExoCarta_8720
Vesiclepedia VP_8720
Entrez Gene 8720
HGNC 15456
MIM 603355
UniProt Q14703  
 MBTPS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
 Gene ontology annotations for MBTPS1
Molecular Function
    serine-type endopeptidase activity GO:0004252 EXP
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 IMP
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IMP
    protein import into nucleus GO:0006606 IEA
    lysosome organization GO:0007040 IMP
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IEA
    cholesterol metabolic process GO:0008203 IEA
    protein processing GO:0016485 IDA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    membrane protein intracellular domain proteolysis GO:0031293 ISS
    response to endoplasmic reticulum stress GO:0034976 ISS
    ATF6-mediated unfolded protein response GO:0036500 TAS
    regulation of cholesterol biosynthetic process GO:0045540 TAS
    protein maturation GO:0051604 IDA
    protein maturation GO:0051604 IMP
    regulation of vesicle-mediated transport GO:0060627 IMP
Subcellular Localization
    Golgi membrane GO:0000139 EXP
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi stack GO:0005795 IEA
 Experiment description of studies that identified MBTPS1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for MBTPS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 C7orf34  
Affinity Capture-MS Homo sapiens
3 GPIHBP1  
Affinity Capture-MS Homo sapiens
4 HSPA5 3309
Affinity Capture-MS Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 NAAA  
Affinity Capture-MS Homo sapiens
8 CEACAM8 1088
Affinity Capture-MS Homo sapiens
9 HLA-G 3135
Affinity Capture-MS Homo sapiens
10 CLGN 1047
Affinity Capture-MS Homo sapiens
11 SIAE 54414
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C11orf58  
Two-hybrid Homo sapiens
14 SPHK1 8877
Biochemical Activity Homo sapiens
15 LRRC59 55379
Proximity Label-MS Homo sapiens
16 PEX19 5824
Affinity Capture-MS Homo sapiens
17 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GGH 8836
Affinity Capture-MS Homo sapiens
19 TNFSF11  
Two-hybrid Homo sapiens
20 E2F1 1869
Negative Genetic Homo sapiens
21 PTCH1  
Affinity Capture-MS Homo sapiens
22 CDK2AP2  
Two-hybrid Homo sapiens
23 PDGFRA 5156
Affinity Capture-MS Homo sapiens
24 PRG2  
Affinity Capture-MS Homo sapiens
25 MFAP4 4239
Affinity Capture-MS Homo sapiens
26 ECEL1  
Affinity Capture-MS Homo sapiens
27 EGFR 1956
Negative Genetic Homo sapiens
28 JMJD8  
Affinity Capture-MS Homo sapiens
29 HDAC1 3065
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
30 GAA 2548
Affinity Capture-MS Homo sapiens
31 NCR3  
Affinity Capture-MS Homo sapiens
32 IL5RA  
Affinity Capture-MS Homo sapiens
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 SFTPC  
Affinity Capture-MS Homo sapiens
35 CLEC12B  
Affinity Capture-MS Homo sapiens
36 BTNL2  
Affinity Capture-MS Homo sapiens
37 C12orf49  
Negative Genetic Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 FIGF 2277
Affinity Capture-MS Homo sapiens
39 MOV10 4343
Affinity Capture-RNA Homo sapiens
40 SDF2L1 23753
Affinity Capture-MS Homo sapiens
41 SP3  
Affinity Capture-MS Homo sapiens
42 SCGB2A2  
Affinity Capture-MS Homo sapiens
43 C1orf54  
Affinity Capture-MS Homo sapiens
44 TRGV3  
Affinity Capture-MS Homo sapiens
45 DNAJB9 4189
Affinity Capture-MS Homo sapiens
46 ZBED8  
Two-hybrid Homo sapiens
47 HDAC2 3066
Affinity Capture-Western Homo sapiens
48 CLEC11A 6320
Affinity Capture-MS Homo sapiens
49 GML  
Affinity Capture-MS Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 ISLR 3671
Affinity Capture-MS Homo sapiens
52 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TNFRSF14  
Two-hybrid Homo sapiens
54 PAGE1  
Affinity Capture-MS Homo sapiens
55 DKKL1  
Affinity Capture-MS Homo sapiens
56 BIRC3 330
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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