Gene description for ALDH3A1
Gene name aldehyde dehydrogenase 3 family, member A1
Gene symbol ALDH3A1
Other names/aliases ALDH3
ALDHIII
Species Homo sapiens
 Database cross references - ALDH3A1
ExoCarta ExoCarta_218
Entrez Gene 218
HGNC 405
MIM 100660
UniProt P30838  
 ALDH3A1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ALDH3A1
Molecular Function
    aldehyde dehydrogenase [NAD(P)+] activity GO:0004030 IEA
    benzaldehyde dehydrogenase (NAD+) activity GO:0018479 IDA
    3-chloroallyl aldehyde dehydrogenase activity GO:0004028 IBA
    alcohol dehydrogenase (NADP+) activity GO:0008106 IDA
    aldehyde dehydrogenase (NAD) activity GO:0004029 IDA
Biological Process
    aging GO:0007568 IEA
    cellular aldehyde metabolic process GO:0006081 IDA
    response to cAMP GO:0051591 IEA
    response to hypoxia GO:0001666 IEA
    response to glucocorticoid GO:0051384 IEA
    response to drug GO:0042493 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    response to nutrient GO:0007584 IEA
    oxidation-reduction process GO:0055114 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 ISS
 Experiment description of studies that identified ALDH3A1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ALDH3A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ALDH3A1 218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ALDH3A1 is involved
No pathways found





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