Gene description for MGAT4B
Gene name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Gene symbol MGAT4B
Other names/aliases GNT-IV
GNT-IVB
Species Homo sapiens
 Database cross references - MGAT4B
ExoCarta ExoCarta_11282
Entrez Gene 11282
HGNC 7048
MIM 604561
UniProt Q9UQ53  
 MGAT4B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MGAT4B
Molecular Function
    alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity GO:0008454 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    N-glycan processing GO:0006491 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    post-translational protein modification GO:0043687 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified MGAT4B in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MGAT4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MGAT4B is involved
PathwayEvidenceSource
N-Glycan antennae elongation TAS Reactome





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