Gene description for MGAT4B
Gene name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Gene symbol MGAT4B
Other names/aliases GNT-IV
GNT-IVB
Species Homo sapiens
 Database cross references - MGAT4B
ExoCarta ExoCarta_11282
Vesiclepedia VP_11282
Entrez Gene 11282
HGNC 7048
MIM 604561
UniProt Q9UQ53  
 MGAT4B identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MGAT4B
Molecular Function
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 IBA
    alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity GO:0008454 IDA
    alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity GO:0008454 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    protein glycosylation GO:0006486 IDA
    protein N-linked glycosylation GO:0006487 IBA
    N-glycan processing GO:0006491 TAS
    viral protein processing GO:0019082 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    Golgi stack GO:0005795 IBA
 Experiment description of studies that identified MGAT4B in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MGAT4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GALNT10 55568
Affinity Capture-MS Homo sapiens
2 HBB 3043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UQCRFS1 7386
Co-fractionation Homo sapiens
4 CEACAM8 1088
Affinity Capture-MS Homo sapiens
5 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SLC26A2 1836
Co-fractionation Homo sapiens
7 TMEM160 54958
Affinity Capture-MS Homo sapiens
8 OPRL1 4987
Affinity Capture-MS Homo sapiens
9 DDX58 23586
Affinity Capture-RNA Homo sapiens
10 SYNJ2BP 55333
Co-fractionation Homo sapiens
11 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
12 SEC62 7095
Affinity Capture-MS Homo sapiens
13 HSPA2 3306
Affinity Capture-MS Homo sapiens
14 SCGB2A2  
Affinity Capture-MS Homo sapiens
15 IGF2R 3482
Affinity Capture-MS Homo sapiens
16 KIR2DS3  
Affinity Capture-MS Homo sapiens
17 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 C3orf52  
Affinity Capture-MS Homo sapiens
19 SFXN2  
Co-fractionation Homo sapiens
20 VDAC1 7416
Co-fractionation Homo sapiens
21 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SLC3A2 6520
Affinity Capture-MS Homo sapiens
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