Gene description for MBD5
Gene name methyl-CpG binding domain protein 5
Gene symbol MBD5
Other names/aliases MRD1
Species Homo sapiens
 Database cross references - MBD5
ExoCarta ExoCarta_55777
Entrez Gene 55777
HGNC 20444
MIM 611472
UniProt Q9P267  
 MBD5 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 19056867    
 Gene ontology annotations for MBD5
Molecular Function
    chromatin binding GO:0003682 IDA
    DNA binding GO:0003677 IDA
Biological Process
    glucose homeostasis GO:0042593 ISS
    regulation of multicellular organism growth GO:0040014 ISS
    single-organism behavior GO:0044708 IMP
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    positive regulation of growth hormone receptor signaling pathway GO:0060399 ISS
    nervous system development GO:0007399 IMP
    positive regulation of defense response to virus by host GO:0002230 IMP
Subcellular Localization
    chromocenter GO:0010369 IDA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified MBD5 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MBD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MBD5 is involved
No pathways found





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