Gene description for MBD5
Gene name methyl-CpG binding domain protein 5
Gene symbol MBD5
Other names/aliases MRD1
Species Homo sapiens
 Database cross references - MBD5
ExoCarta ExoCarta_55777
Vesiclepedia VP_55777
Entrez Gene 55777
HGNC 20444
MIM 611472
UniProt Q9P267  
 MBD5 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 19056867    
 Gene ontology annotations for MBD5
Molecular Function
    DNA binding GO:0003677 IDA
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    protein binding GO:0005515 IPI
Biological Process
    nervous system development GO:0007399 IMP
    regulation of multicellular organism growth GO:0040014 ISS
    glucose homeostasis GO:0042593 ISS
    regulation of behavior GO:0050795 IMP
    positive regulation of growth hormone receptor signaling pathway GO:0060399 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IEA
    chromocenter GO:0010369 IBA
    chromocenter GO:0010369 IDA
    midbody GO:0030496 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MBD5 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MBD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BID  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RHOA 387
Affinity Capture-MS Homo sapiens
3 APC  
Two-hybrid Homo sapiens
4 NAA15 80155
Affinity Capture-MS Homo sapiens
5 KIAA1549  
Two-hybrid Homo sapiens
6 NME2P1 283458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SNX3 8724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 UBA6 55236
Affinity Capture-MS Homo sapiens
10 ASXL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 UBE2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NAA50 80218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UBE2E1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ETF1 2107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PMVK 10654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LZIC 84328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SCRN1 9805
Affinity Capture-MS Homo sapiens
18 BAP1 8314
Affinity Capture-MS Homo sapiens
19 EIF2S2 8894
Affinity Capture-MS Homo sapiens
20 HMGCS1 3157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MBD5 is involved
PathwayEvidenceSource
Deubiquitination TAS Reactome
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
UCH proteinases TAS Reactome





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