Gene description for ZCCHC11
Gene name zinc finger, CCHC domain containing 11
Gene symbol ZCCHC11
Other names/aliases PAPD3
TUT4
Species Homo sapiens
 Database cross references - ZCCHC11
ExoCarta ExoCarta_23318
Vesiclepedia VP_23318
Entrez Gene 23318
HGNC 28981
MIM 613692
UniProt Q5TAX3  
 ZCCHC11 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for ZCCHC11
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    miRNA binding GO:0035198 ISS
    RNA uridylyltransferase activity GO:0050265 IBA
    RNA uridylyltransferase activity GO:0050265 IDA
Biological Process
    oocyte maturation GO:0001556 ISS
    miRNA metabolic process GO:0010586 IDA
    miRNA metabolic process GO:0010586 ISS
    miRNA catabolic process GO:0010587 IMP
    stem cell population maintenance GO:0019827 IMP
    pre-miRNA processing GO:0031054 IDA
    pre-miRNA processing GO:0031054 IMP
    RNA 3'-end processing GO:0031123 IDA
    histone mRNA catabolic process GO:0071044 IMP
    RNA 3' uridylation GO:0071076 IBA
    RNA 3' uridylation GO:0071076 ISS
    transposable element silencing by mRNA destabilization GO:0141008 IDA
    polyuridylation-dependent mRNA catabolic process GO:1990074 ISS
Subcellular Localization
    extracellular space GO:0005615 HDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ZCCHC11 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ZCCHC11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CUL1 8454
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 TIFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 EGLN3  
Affinity Capture-MS Homo sapiens
6 BAG2 9532
Affinity Capture-MS Homo sapiens
7 CUL5 8065
Affinity Capture-MS Homo sapiens
8 PHIP 55023
Affinity Capture-MS Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 SQSTM1 8878
Proximity Label-MS Homo sapiens
11 MIR363  
Affinity Capture-RNA Homo sapiens
12 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 DDX58 23586
Affinity Capture-RNA Homo sapiens
15 YBX2 51087
Two-hybrid Homo sapiens
16 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
17 CUL4B 8450
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 FKBP5 2289
Affinity Capture-MS Homo sapiens
20 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
21 MYC  
Affinity Capture-MS Homo sapiens
22 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
23 COPS5 10987
Affinity Capture-MS Homo sapiens
24 CPSF7 79869
Two-hybrid Homo sapiens
25 FBL 2091
Proximity Label-MS Homo sapiens
26 CUL2 8453
Affinity Capture-MS Homo sapiens
27 DUSP16  
Affinity Capture-MS Homo sapiens
28 RHOT2 89941
Proximity Label-MS Homo sapiens
29 MIRLET7A1  
Affinity Capture-RNA Homo sapiens
30 EXD2  
Proximity Label-MS Homo sapiens
31 CAPN2 824
Affinity Capture-MS Homo sapiens
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