Gene description for ZCCHC11
Gene name zinc finger, CCHC domain containing 11
Gene symbol ZCCHC11
Other names/aliases PAPD3
TUT4
Species Homo sapiens
 Database cross references - ZCCHC11
ExoCarta ExoCarta_23318
Entrez Gene 23318
HGNC 28981
MIM 613692
UniProt Q5TAX3  
 ZCCHC11 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for ZCCHC11
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    RNA uridylyltransferase activity GO:0050265 IDA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
Biological Process
    stem cell maintenance GO:0019827 IMP
    miRNA metabolic process GO:0010586 ISS
    cytokine production GO:0001816 IEA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IEA
    pre-miRNA processing GO:0031054 IMP
    histone mRNA catabolic process GO:0071044 IMP
    RNA 3'-end processing GO:0031123 IDA
    regulation of lipopolysaccharide-mediated signaling pathway GO:0031664 IEA
    positive regulation of interleukin-6 production GO:0032755 IEA
    miRNA catabolic process GO:0010587 IMP
    interleukin-6-mediated signaling pathway GO:0070102 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified ZCCHC11 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ZCCHC11
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ZCCHC11 is involved
No pathways found





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