Gene description for QTRT1
Gene name queuine tRNA-ribosyltransferase 1
Gene symbol QTRT1
Other names/aliases FP3235
TGT
TGUT
Species Homo sapiens
 Database cross references - QTRT1
ExoCarta ExoCarta_81890
Vesiclepedia VP_81890
Entrez Gene 81890
HGNC 23797
MIM 609615
UniProt Q9BXR0  
 QTRT1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for QTRT1
Molecular Function
    protein binding GO:0005515 IPI
    tRNA-guanosine(34) queuine transglycosylase activity GO:0008479 IBA
    tRNA-guanosine(34) queuine transglycosylase activity GO:0008479 IDA
    tRNA-guanosine(34) queuine transglycosylase activity GO:0008479 TAS
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    tRNA modification GO:0006400 TAS
    tRNA-guanine transglycosylation GO:0101030 IBA
    tRNA-guanine transglycosylation GO:0101030 IDA
    tRNA-guanine transglycosylation GO:0101030 NAS
Subcellular Localization
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HTP
    mitochondrial outer membrane GO:0005741 NAS
    mitochondrial outer membrane GO:0005741 TAS
    cytoplasmic side of mitochondrial outer membrane GO:0032473 IEA
    tRNA-guanine transglycosylase complex GO:0120507 IPI
    transferase complex GO:1990234 IPI
 Experiment description of studies that identified QTRT1 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for QTRT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RHOU 58480
Reconstituted Complex Homo sapiens
4 THUMPD1 55623
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HSPA8 3312
Affinity Capture-MS Homo sapiens
6 FAS 355
Affinity Capture-MS Homo sapiens
7 HPCAL1 3241
Affinity Capture-MS Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 NDUFA4L2 56901
Two-hybrid Homo sapiens
10 SH3GL3  
Two-hybrid Homo sapiens
11 ISCA1  
Affinity Capture-MS Homo sapiens
12 GPR107 57720
Affinity Capture-MS Homo sapiens
13 ZBTB16  
Two-hybrid Homo sapiens
14 APBB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SETDB1  
Two-hybrid Homo sapiens
16 APP 351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PRAME  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RPA3 6119
Proximity Label-MS Homo sapiens
19 APBB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 COPS6 10980
Two-hybrid Homo sapiens
21 PRSS8 5652
Affinity Capture-MS Homo sapiens
22 CLEC11A 6320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SERBP1 26135
Affinity Capture-MS Homo sapiens
24 UNC119 9094
Two-hybrid Homo sapiens
25 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NOG 9241
Affinity Capture-MS Homo sapiens
27 ACSL4 2182
Affinity Capture-MS Homo sapiens
28 CDC42EP4  
Affinity Capture-MS Homo sapiens
29 TAS1R2  
Affinity Capture-MS Homo sapiens
30 QTRTD1  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which QTRT1 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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