Gene description for ZNF473
Gene name zinc finger protein 473
Gene symbol ZNF473
Other names/aliases HZFP100
ZN473
Species Homo sapiens
 Database cross references - ZNF473
ExoCarta ExoCarta_25888
Vesiclepedia VP_25888
Entrez Gene 25888
HGNC 23239
UniProt Q8WTR7  
 ZNF473 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ZNF473
Molecular Function
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    mRNA 3'-end processing GO:0031124 TAS
    gene expression GO:0010467 TAS
    histone mRNA metabolic process GO:0008334 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    histone mRNA 3'-end processing GO:0006398 IDA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    Cajal body GO:0015030 IDA
 Experiment description of studies that identified ZNF473 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ZNF473
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLBP  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRIM41 90933
Two-hybrid Homo sapiens
3 LSM11  
Reconstituted Complex Homo sapiens
4 ZNF202  
Two-hybrid Homo sapiens
View the network image/svg+xml



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