Gene description for RHPN2
Gene name rhophilin, Rho GTPase binding protein 2
Gene symbol RHPN2
Other names/aliases P76RBE
RHOBP
Species Homo sapiens
 Database cross references - RHPN2
ExoCarta ExoCarta_85415
Entrez Gene 85415
HGNC 19974
UniProt Q8IUC4  
 RHPN2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RHPN2
Biological Process
    small GTPase mediated signal transduction GO:0007264 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified RHPN2 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RHPN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 KRT18 3875
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 RHOB 388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 CNKSR1  
Affinity Capture-Western Homo sapiens
5 RHOA 387
Two-hybrid Homo sapiens
6 TCF1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RHPN2 is involved
PathwayEvidenceSource
RHO GTPases Activate Rhotekin and Rhophilins TAS Reactome





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