Gene description for DLC1
Gene name DLC1 Rho GTPase activating protein
Gene symbol DLC1
Other names/aliases ARHGAP7
HP
STARD12
p122-RhoGAP
Species Homo sapiens
 Database cross references - DLC1
ExoCarta ExoCarta_10395
Vesiclepedia VP_10395
Entrez Gene 10395
HGNC 2897
MIM 604258
UniProt Q96QB1  
 DLC1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for DLC1
Molecular Function
    SH2 domain binding GO:0042169 IPI
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IMP
    lipid binding GO:0008289 IEA
Biological Process
    regulation of actin cytoskeleton organization GO:0032956 IMP
    apoptotic process GO:0006915 IDA
    forebrain development GO:0030900 ISS
    positive regulation of GTPase activity GO:0043547 IMP
    neural tube closure GO:0001843 ISS
    negative regulation of Rho protein signal transduction GO:0035024 IMP
    focal adhesion assembly GO:0048041 ISS
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of execution phase of apoptosis GO:1900119 IDA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of protein dephosphorylation GO:0035307 IDA
    negative regulation of cell migration GO:0030336 IMP
    negative regulation of focal adhesion assembly GO:0051895 IDA
    negative regulation of stress fiber assembly GO:0051497 IDA
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of cell shape GO:0008360 IMP
    hindbrain morphogenesis GO:0021575 ISS
    heart morphogenesis GO:0003007 ISS
    negative regulation of cell proliferation GO:0008285 IMP
Subcellular Localization
    ruffle membrane GO:0032587 IDA
    nucleus GO:0005634 IDA
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    cortical actin cytoskeleton GO:0030864 IDA
    caveola GO:0005901 IDA
 Experiment description of studies that identified DLC1 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DLC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DLC1 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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