Gene description for KLKB1
Gene name kallikrein B, plasma (Fletcher factor) 1
Gene symbol KLKB1
Other names/aliases KLK3
PPK
Species Homo sapiens
 Database cross references - KLKB1
ExoCarta ExoCarta_3818
Vesiclepedia VP_3818
Entrez Gene 3818
HGNC 6371
MIM 229000
UniProt P03952  
 KLKB1 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for KLKB1
Molecular Function
    serine-type endopeptidase activity GO:0004252 IEA
    protein binding GO:0005515 IPI
Biological Process
    Factor XII activation GO:0002542 TAS
    proteolysis GO:0006508 TAS
    blood coagulation GO:0007596 IEA
    zymogen activation GO:0031638 TAS
    plasminogen activation GO:0031639 IDA
    fibrinolysis GO:0042730 IEA
    positive regulation of fibrinolysis GO:0051919 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KLKB1 in exosomes
1
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for KLKB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NID2 22795
Affinity Capture-MS Homo sapiens
2 TOR3A 64222
Affinity Capture-MS Homo sapiens
3 SERPINA5 5104
Reconstituted Complex Homo sapiens
4 NCAPH 23397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CNOT2  
Affinity Capture-MS Homo sapiens
6 FBN2 2201
Affinity Capture-MS Homo sapiens
7 GPHN 10243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ZBTB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KNG1 3827
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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