Gene description for C9orf116
Gene name chromosome 9 open reading frame 116
Gene symbol C9orf116
Other names/aliases PIERCE1
RbEST47
Species Homo sapiens
 Database cross references - C9orf116
ExoCarta ExoCarta_138162
Vesiclepedia VP_138162
Entrez Gene 138162
HGNC 28435
MIM 614502
UniProt Q5BN46  
 C9orf116 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for C9orf116
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cilium movement GO:0003341 ISS
    DNA damage response GO:0006974 IEA
    determination of left/right symmetry GO:0007368 ISS
    regulation of gene expression GO:0010468 IEA
    flagellated sperm motility GO:0030317 ISS
    axoneme assembly GO:0035082 ISS
    establishment of left/right asymmetry GO:0061966 ISS
    cellular response to UV-C GO:0071494 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
    axonemal microtubule GO:0005879 IDA
    axonemal microtubule GO:0005879 ISS
    sperm flagellum GO:0036126 ISS
    axonemal A tubule inner sheath GO:0160111 ISS
 Experiment description of studies that identified C9orf116 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for C9orf116
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FHL3 2275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 HSPA8 3312
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which C9orf116 is involved
No pathways found





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