Gene description for Rps6ka3
Gene name ribosomal protein S6 kinase polypeptide 3
Gene symbol Rps6ka3
Other names/aliases C130006E23
MAPKAPK-1b
MPK-9
Rsk2
S6K-alpha3
p90RSK3
pp90RSK2
Species Mus musculus
 Database cross references - Rps6ka3
ExoCarta ExoCarta_110651
Vesiclepedia VP_110651
Entrez Gene 110651
UniProt P18654  
 Rps6ka3 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps6ka3
Molecular Function
    protein kinase activity GO:0004672 IEA
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISO
    ribosomal protein S6 kinase activity GO:0004711 IBA
    transferase activity GO:0016740 IEA
    nucleotide binding GO:0000166 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    kinase activity GO:0016301 IEA
    ATP binding GO:0005524 IEA
    magnesium ion binding GO:0000287 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    intracellular signal transduction GO:0035556 IBA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    toll-like receptor signaling pathway GO:0002224 IMP
    phosphorylation GO:0016310 IEA
    response to lipopolysaccharide GO:0032496 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    regulation of translation GO:0006417 IBA
    cell cycle GO:0007049 IEA
    protein phosphorylation GO:0006468 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified Rps6ka3 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps6ka3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Affinity Capture-Western Homo sapiens
2 MAPK3 5595
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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