Gene description for Dlg1
Gene name discs, large homolog 1 (Drosophila)
Gene symbol Dlg1
Other names/aliases B130052P05Rik
Dlgh1
E-dlg/SAP97
SAP-97
SAP97
mKIAA4187
Species Mus musculus
 Database cross references - Dlg1
ExoCarta ExoCarta_13383
Vesiclepedia VP_13383
Entrez Gene 13383
UniProt Q811D0  
 Dlg1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dlg1
Molecular Function
    potassium channel regulator activity GO:0015459 ISO
    PDZ domain binding GO:0030165 ISO
    L27 domain binding GO:0097016 ISO
    protein binding GO:0005515 IPI
    guanylate kinase activity GO:0004385 IBA
    kinase binding GO:0019900 ISO
    ion channel binding GO:0044325 ISO
    mitogen-activated protein kinase kinase binding GO:0031434 ISO
    protein C-terminus binding GO:0008022 ISO
    protein kinase binding GO:0019901 ISO
    ionotropic glutamate receptor binding GO:0035255 ISO
    phosphatase binding GO:0019902 ISO
    protein complex scaffold GO:0032947 IMP
Biological Process
    bicellular tight junction assembly GO:0070830 ISO
    cortical actin cytoskeleton organization GO:0030866 ISO
    single organismal cell-cell adhesion GO:0016337 ISO
    positive regulation of cell proliferation GO:0008284 IMP
    nucleotide phosphorylation GO:0046939 IBA
    negative regulation of mitotic cell cycle GO:0045930 ISO
    regulation of membrane potential GO:0042391 ISO
    nervous system development GO:0007399 IBA
    lens development in camera-type eye GO:0002088 IMP
    receptor localization to synapse GO:0097120 IBA
    ureteric bud development GO:0001657 IMP
    membrane raft organization GO:0031579 IMP
    synaptic transmission GO:0007268 IBA
    reproductive structure development GO:0048608 IMP
    positive regulation of potassium ion transport GO:0043268 ISO
    actin filament organization GO:0007015 ISO
    protein localization GO:0008104 IMP
    activation of protein kinase activity GO:0032147 IMP
    amyloid precursor protein metabolic process GO:0042982 IGI
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    negative regulation of protein kinase B signaling GO:0051898 IMP
    protein localization to plasma membrane GO:0072659 ISO
    tissue morphogenesis GO:0048729 IMP
    endothelial cell proliferation GO:0001935 ISO
    hard palate development GO:0060022 IMP
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    negative regulation of T cell proliferation GO:0042130 IMP
    T cell cytokine production GO:0002369 IMP
    T cell activation GO:0042110 IMP
    peristalsis GO:0030432 IMP
    smooth muscle tissue development GO:0048745 IMP
    regulation of protein localization GO:0032880 ISO
    positive regulation of actin filament polymerization GO:0030838 IMP
    regulation of myelination GO:0031641 IMP
    positive regulation of multicellular organism growth GO:0040018 TAS
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISO
    embryonic skeletal system morphogenesis GO:0048704 IMP
    immunological synapse formation GO:0001771 IMP
    receptor clustering GO:0043113 IBA
    branching involved in ureteric bud morphogenesis GO:0001658 IMP
    positive regulation of developmental growth GO:0048639 TAS
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    myelin sheath abaxonal region GO:0035748 IDA
    microtubule GO:0005874 ISO
    bicellular tight junction GO:0005923 ISO
    postsynaptic membrane GO:0045211 IBA
    cell-cell adherens junction GO:0005913 TAS
    nucleus GO:0005634 ISO
    plasma membrane GO:0005886 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    immunological synapse GO:0001772 IDA
    Golgi apparatus GO:0005794 ISO
    T-tubule GO:0030315 ISO
    lateral plasma membrane GO:0016328 IDA
    membrane raft GO:0045121 IDA
    basal lamina GO:0005605 IDA
    basal plasma membrane GO:0009925 ISO
    MPP7-DLG1-LIN7 complex GO:0097025 ISO
    lateral loop GO:0043219 IDA
    synapse GO:0045202 IDA
    cell junction GO:0030054 ISO
    postsynaptic density GO:0014069 ISO
    neuromuscular junction GO:0031594 IDA
    neuron projection GO:0043005 ISO
    cytoplasm GO:0005737 ISO
    basolateral plasma membrane GO:0016323 TAS
    cell projection membrane GO:0031253 IDA
    cell-cell junction GO:0005911 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    endoplasmic reticulum GO:0005783 ISO
    node of Ranvier GO:0033268 IDA
    presynaptic membrane GO:0042734 ISO
    ionotropic glutamate receptor complex GO:0008328 IBA
 Experiment description of studies that identified Dlg1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dlg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dlg1 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor IEA Reactome
NrCAM interactions IEA Reactome





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