Gene description for Zap70
Gene name zeta-chain (TCR) associated protein kinase
Gene symbol Zap70
Other names/aliases Srk
ZAP-70
mrtle
mur
Species Mus musculus
 Database cross references - Zap70
ExoCarta ExoCarta_22637
Vesiclepedia VP_22637
Entrez Gene 22637
UniProt P43404  
 Zap70 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Zap70
Molecular Function
    phosphotyrosine residue binding GO:0001784 IPI
    phosphotyrosine residue binding GO:0001784 ISO
    protein kinase activity GO:0004672 IDA
    protein tyrosine kinase activity GO:0004713 IBA
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 IMP
    protein tyrosine kinase activity GO:0004713 ISO
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    adaptive immune response GO:0002250 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    immune response GO:0006955 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    calcium-mediated signaling GO:0019722 IMP
    T cell differentiation GO:0030217 IMP
    intracellular signal transduction GO:0035556 IMP
    beta selection GO:0043366 IGI
    positive thymic T cell selection GO:0045059 IMP
    positive thymic T cell selection GO:0045059 ISO
    negative thymic T cell selection GO:0045060 IMP
    thymic T cell selection GO:0045061 IMP
    positive regulation of T cell differentiation GO:0045582 IMP
    positive regulation of T cell differentiation GO:0045582 ISO
    alpha-beta T cell differentiation GO:0046632 IGI
    alpha-beta T cell differentiation GO:0046632 IMP
    positive regulation of alpha-beta T cell differentiation GO:0046638 IGI
    positive regulation of alpha-beta T cell differentiation GO:0046638 IMP
    positive regulation of alpha-beta T cell proliferation GO:0046641 IGI
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 IMP
    positive regulation of calcium-mediated signaling GO:0050850 IMP
    T cell receptor signaling pathway GO:0050852 IMP
    T cell receptor signaling pathway GO:0050852 ISO
Subcellular Localization
    immunological synapse GO:0001772 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    membrane GO:0016020 IDA
    T cell receptor complex GO:0042101 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Zap70 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Zap70
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lck 16818
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Slc4a1  
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
3 Sla2 77799
Affinity Capture-Western Mus musculus
4 PELI1 57162
Reconstituted Complex Homo sapiens
5 Crkl 12929
Affinity Capture-Western Mus musculus
6 Cd247  
Affinity Capture-Western Mus musculus
7 Dlg1 13383
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Sla 20491
Affinity Capture-Western Mus musculus
9 Vav1 22324
Affinity Capture-Western Mus musculus
10 Cbl 12402
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
11 Was 22376
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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