Gene description for Adam10
Gene name a disintegrin and metallopeptidase domain 10
Gene symbol Adam10
Other names/aliases 1700031C13Rik
MADM
kuz
kuzbanian
Species Mus musculus
 Database cross references - Adam10
ExoCarta ExoCarta_11487
Vesiclepedia VP_11487
Entrez Gene 11487
UniProt O35598  
 Adam10 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Plasma 32132711    
Plasma 32132711    
Plasma 32132711    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adam10
Molecular Function
    zinc ion binding GO:0008270 IEA
    protein kinase binding GO:0019901 IDA
    protein homodimerization activity GO:0042803 IPI
    metal ion binding GO:0046872 IEA
    SH2 domain binding GO:0042169 NAS
    hydrolase activity GO:0016787 IEA
    endopeptidase activity GO:0004175 IDA
    metalloendopeptidase activity GO:0004222 ISO
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    metallopeptidase activity GO:0008237 ISO
    peptidase activity GO:0008233 IEA
Biological Process
    in utero embryonic development GO:0001701 IMP
    proteolysis GO:0006508 IMP
    positive regulation of cell proliferation GO:0008284 ISO
    nucleocytoplasmic transport GO:0006913 NAS
    membrane protein ectodomain proteolysis GO:0006509 ISO
    positive regulation of cell growth GO:0030307 ISO
    constitutive protein ectodomain proteolysis GO:0051089 ISO
    response to tumor necrosis factor GO:0034612 ISO
    positive regulation of cell migration GO:0030335 ISO
    protein phosphorylation GO:0006468 IDA
    Notch receptor processing GO:0007220 NAS
    negative regulation of cell adhesion GO:0007162 ISO
    Notch signaling pathway GO:0007219 IMP
    monocyte activation GO:0042117 ISO
    PMA-inducible membrane protein ectodomain proteolysis GO:0051088 ISO
    positive regulation of T cell chemotaxis GO:0010820 ISO
Subcellular Localization
    Golgi-associated vesicle GO:0005798 ISO
    Golgi apparatus GO:0005794 ISO
    perinuclear endoplasmic reticulum GO:0097038 ISO
    trans-Golgi network GO:0005802 ISO
    cytoplasm GO:0005737 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    nucleus GO:0005634 IDA
    postsynaptic density GO:0014069 IDA
    cell GO:0005623 NAS
    cell surface GO:0009986 ISO
    extracellular exosome GO:0070062 ISO
    focal adhesion GO:0005925 ISO
    membrane GO:0016020 ISO
    intracellular GO:0005622 NAS
    integral component of membrane GO:0016021 IEA
    tetraspanin-enriched microdomain GO:0097197 ISO
 Experiment description of studies that identified Adam10 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 333
MISEV standards
✘ EV Biophysical techniques
✔
Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K.
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 334
MISEV standards
✘ EV Biophysical techniques
✔
Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K.
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 335
MISEV standards
✘ EV Biophysical techniques
✔
Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K.
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adam10
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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