Gene description for Cask
Gene name calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Gene symbol Cask
Other names/aliases DXPri1
DXRib1
LIN-2
Pals3
mLin-2
Species Mus musculus
 Database cross references - Cask
ExoCarta ExoCarta_12361
Vesiclepedia VP_12361
Entrez Gene 12361
UniProt O70589  
 Cask identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cask
Molecular Function
    protein kinase activity GO:0004672 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    nucleotide binding GO:0000166 IEA
    ATP binding GO:0005524 IEA
    protein C-terminus binding GO:0008022 ISO
    protein binding GO:0005515 IPI
    kinase activity GO:0016301 IEA
    calmodulin binding GO:0005516 IEA
    protein complex binding GO:0032403 ISO
    PDZ domain binding GO:0030165 ISO
    transferase activity GO:0016740 IEA
    neurexin family protein binding GO:0042043 ISO
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
Biological Process
    positive regulation of calcium ion import GO:0090280 IDA
    intracellular protein transport GO:0006886 ISO
    phosphorylation GO:0016310 IEA
    negative regulation of cellular response to growth factor stimulus GO:0090288 ISO
    negative regulation of wound healing GO:0061045 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    negative regulation of keratinocyte proliferation GO:0010839 ISO
    protein phosphorylation GO:0006468 IEA
    negative regulation of cell-matrix adhesion GO:0001953 ISO
    calcium ion import GO:0070509 IMP
Subcellular Localization
    plasma membrane GO:0005886 ISO
    vesicle GO:0031982 IDA
    presynaptic membrane GO:0042734 ISO
    membrane GO:0016020 ISO
    nuclear lamina GO:0005652 ISO
    ciliary membrane GO:0060170 ISO
    nucleolus GO:0005730 ISO
    cytosol GO:0005829 IDA
    synapse GO:0045202 IDA
    cell-cell junction GO:0005911 ISO
    basement membrane GO:0005604 ISO
    cytoplasm GO:0005737 ISO
    nuclear matrix GO:0016363 ISO
    synaptic membrane GO:0097060 ISO
    basolateral plasma membrane GO:0016323 IDA
    dendrite GO:0030425 ISO
    protein complex GO:0043234 ISO
    focal adhesion GO:0005925 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Cask in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cask
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cask is involved
PathwayEvidenceSource
Dopamine Neurotransmitter Release Cycle IEA Reactome
Nephrin interactions IEA Reactome
Syndecan interactions IEA Reactome





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