Gene description for SPINK1
Gene name serine peptidase inhibitor, Kazal type 1
Gene symbol SPINK1
Other names/aliases PCTT
PSTI
Spink3
TATI
TCP
Species Homo sapiens
 Database cross references - SPINK1
ExoCarta ExoCarta_6690
Entrez Gene 6690
HGNC 11244
MIM 167790
UniProt P00995  
 SPINK1 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for SPINK1
Molecular Function
    endopeptidase inhibitor activity GO:0004866 TAS
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IEA
    regulation of acrosome reaction GO:0060046 IEA
    regulation of store-operated calcium entry GO:2001256 IEA
    negative regulation of serine-type endopeptidase activity GO:1900004 IEA
    negative regulation of calcium ion import GO:0090281 IEA
    negative regulation of nitric oxide mediated signal transduction GO:0010751 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IEA
 Experiment description of studies that identified SPINK1 in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for SPINK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPINK1 is involved
No pathways found





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