Gene description for Lsm7
Gene name LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Gene symbol Lsm7
Other names/aliases 0910001B06Rik
1110033F18Rik
Species Mus musculus
 Database cross references - Lsm7
ExoCarta ExoCarta_66094
Vesiclepedia VP_66094
Entrez Gene 66094
UniProt Q9CQQ8  
 Lsm7 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Lsm7
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    nuclear-transcribed mRNA catabolic process GO:0000956 IEA
    mRNA catabolic process GO:0006402 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    U6 snRNP GO:0005688 IBA
    U12-type spliceosomal complex GO:0005689 IBA
    cytoplasm GO:0005737 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISS
    U2-type prespliceosome GO:0071004 IBA
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    spliceosomal tri-snRNP complex GO:0097526 IBA
    Lsm2-8 complex GO:0120115 ISO
    Lsm2-8 complex GO:0120115 ISS
    Lsm1-7-Pat1 complex GO:1990726 IBA
 Experiment description of studies that identified Lsm7 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Lsm7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Thoc1  
Co-fractionation Mus musculus
4 Prpf40a  
Co-fractionation Mus musculus
5 Cactin  
Co-fractionation Mus musculus
6 Snrnp27  
Co-fractionation Mus musculus
7 Usp15  
Affinity Capture-MS Mus musculus
8 Tfip11  
Co-fractionation Mus musculus
9 Zmat2  
Co-fractionation Mus musculus
10 Cdc5l 71702
Co-fractionation Mus musculus
11 Smu1  
Co-fractionation Mus musculus
12 Ppwd1  
Co-fractionation Mus musculus
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