Gene description for Ddx6
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Gene symbol Ddx6
Other names/aliases 1110001P04Rik
E230023J21Rik
HLR2
mRCK/P54
p54
rck
Species Mus musculus
 Database cross references - Ddx6
ExoCarta ExoCarta_13209
Entrez Gene 13209
UniProt P54823  
 Ddx6 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddx6
Molecular Function
    nucleic acid binding GO:0003676 IEA
    RNA binding GO:0003723 IEA
    hydrolase activity GO:0016787 IEA
    nucleotide binding GO:0000166 IEA
    protein domain specific binding GO:0019904 ISO
    ATP binding GO:0005524 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    poly(A) RNA binding GO:0044822 ISO
    helicase activity GO:0004386 IEA
Biological Process
    RNA secondary structure unwinding GO:0010501 IBA
    viral RNA genome packaging GO:0019074 ISO
    negative regulation of neuron differentiation GO:0045665 IMP
    regulation of translation GO:0006417 IBA
    cytoplasmic mRNA processing body assembly GO:0033962 ISO
    stem cell maintenance GO:0019827 IMP
Subcellular Localization
    cytoplasmic mRNA processing body GO:0000932 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasm GO:0005737 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    membrane GO:0016020 ISO
    RISC complex GO:0016442 ISO
 Experiment description of studies that identified Ddx6 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx6 is involved
PathwayEvidenceSource
mRNA decay by 5' to 3' exoribonuclease IEA Reactome





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