Gene description for Arhgef1
Gene name Rho guanine nucleotide exchange factor (GEF) 1
Gene symbol Arhgef1
Other names/aliases Lbcl2
Lsc
Species Mus musculus
 Database cross references - Arhgef1
ExoCarta ExoCarta_16801
Entrez Gene 16801
UniProt Q61210  
 Arhgef1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Arhgef1
Molecular Function
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IEA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    regulation of Rho protein signal transduction GO:0035023 IEA
    negative regulation of cell death GO:0060548 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Arhgef1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Arhgef1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMURF2  
Affinity Capture-Luminescence Homo sapiens
2 SMAD2 4087
Affinity Capture-Luminescence Homo sapiens
3 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Arhgef1 is involved
PathwayEvidenceSource
Axonal growth inhibition (RHOA activation) IEA Reactome
G alpha (12/13) signalling events IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here